Journal
of IMAB
AnnualProceeding
(Scientific Papers)
ISSN: 1312-773X (Online)
Issue: 2005, Book 1
CONTENTS:
COMPARISON OF THE SENSITIVITY OF THE POLIMERASE
CHAIN REACTION FOR DETECTION OF CHLAMYDIA PNEUMONIAE INFECTIONS AFTER
TWO DIFFERENT APPLIED METHODS FOR DNA EXTRACTION FROM SPUTA, TRACHEAL
AND BRONCHIAL SECRETS
V. Ouzounova, I.
Mitov
Department of Microbiology, Medical Faculty, Medical University-Sofia;
SUMMARY: Chlamydia pneumoniae (Chlamydophila pneumoniae)
is one of the important pathogens in human respiratory tract infections.
The microogranism is an obligatory intracellular gram-negative bacterium
that is common cause of bronchitis, sinusitis, pharyngitis and atypical
pneumonia. 90% or more of C. pneumoniae infections remain asymptomatic.
There is suggested a relationship between C. pneumoniae and chronic diseases
such as chronic obstructive pulmonary disease, coronary heart disease,
multiple sclerosis, reactive arthritis, Guillain-Barre’ syndrome, Alzheimer’s
disease, etc. The knowledge for the epidemiology of C. pneumoniae infections
mainly depends on serological detection received from studies of the population
immune status using microimmunofluorescence and cell culture. These methods
are time consuming and not very sensitive. Recently the isolation of the
microorganism was improved and more frequently the PCR started to be applied
as a method of choice to diagnose C. pneumoniae infections. The method
is performed within several hours and gives the possibility to use a wide
variety of patient’s specimens.
The aim of this study is to compare the sensitivity of the polimerase
chain reaction after two different types of DNA extraction from sputa,
tracheal and bronchial secrets, artificially infected with C. pneumoniae.
Key words: Chlamydia pneumoniae, PCR, phenol/chlorophorm/isoamyl
alcohol isolation, sputum, tracheal and bronchial secrets.
HEMODIAPERFUSION IN LEPTOSPIROSIS
Gancheva G, P.
Ilieva, M. Atanasova, Chr. Tzvetanova, I. Simova
Department of Infectious Diseases, Epidemiology, Parasitology and
Tropical Medicine
Medical University, Pleven, Bulgaria
ABSTRACT: Comparatively have been studied laboratory
parameters in severe cases of leptospirosis divided in two groups – with
and without hemodiaperfusion (HD). The dynamical investigations of blood
urea nitrogen (BUN), serum creatinine, and serum bilirubin levels reveal
that hemodiaperfusion markedly decreases the levels of nitrogen parameters
and is non effective for serum bilirubin. Hemodiaperfusion in oliguric
phase of the acute renal failure of leptospirosis prevents brain edema
and lung edema - significant tanatogenic factors.
Key words: hemodiaperfusion, leptospirosis, acute renal
failure, blood urea nitrogen, serum creatinine, serum bilirubin.
PANCREATIC INVOLVEMENT IN LEPTOSPIROSIS
Atanasova M G. Gancheva, P.
Ilieva, Chr. Tzvetanova, I. Simova
Department of Infectious Diseases, Epidemiology, Parasitology and
Tropical Medicine
Medical University, Pleven, Bulgaria
ABSTRACT: The research includes 84 patients with leptospirosis
treated in Clinic at Infectious Diseases – Pleven. Mild course in 52,38%,
moderate in 19,05%, and severe in 28,57% are established. Serum amylase
level has been investigated in 37 cases. Increased rates are measured
in 41,67% of mild cases (av. 279 U/L), in 25% of moderate cases (av. 290
U/L), and in 88,24% of severe cases(av. 937 U/L). Significant difference
between serum amylase levels of mild and severe cases is established (p
< 0,02). Presumably immunological mechanisms of damage by participation
of interleukins (IL1, IL6, IL8) and tumor necrosis factor (TNF-á) are
in the base of increased serum amylase level in leptospirosis. The functional
changes of pancreas in leptospirosis are not fully clear and further research
is needed.
Key words: leptospirosis, pancreas, serum amylase, interleukins.
TANATOGENESIS IN LEPTOSPIROSIS
Gancheva G, P.
Ilieva, M. Atanasova, Chr. Tzvetanova, I. Simova
Department of Infectious Diseases, Epidemiology, Parasitology and
Tropical Medicine
Medical University, Pleven, Bulgaria
ABSTRACT: During the period 1982-2004 in our clinic 84
patients with leptospirosis have been treated. This study dynamically
compares changes of laboratory parameters in survivors and fatal cases,
and reveals following post mortem founded tanatogenic factors: brain edema
in 100% of non survivors, lung edema (100%), severe cardiovascular disorders
with shock (100%), and hemorrhagic syndrome (80%).
Key words: tanatogenesis, acute renal failure, brain
edema, lung edema, cardiovascular disorders, hemorrhagic syndrome.
RECURRENT ANAPLASTIC MENINGIOMA AFTER SURGERY AND RADIATION THERAPY
FOR BASAL CELL CARCINOMA - CASE REPORT
A. Kaprelyan,
N. Deleva
Neurology department, Medical University – Varna
ABSTRACT: A case-report of 63-year female who developed
meningioma after surgery and contact cranial radiotherapy for basal cell
carcinoma (BCC) was presented. Initial symptoms appeared in a latency
period of 14 years and included moderate frontal and occipital headache,
nausea, speech disorder, gait instability, memory impairment and secondary
generalized seizure. The tumor lesion visualized on the computed tomography
(CT) and the medical history of previous cancer disease led initially
our thinking to the diagnosis of cerebral metastasis. Histological verification
confirmed anaplastic meningioma. Long-term follow-up of clinical, CT and
electrophysiological findings corresponded with the recurrent course of
the disease 10 months after gross-total tumor extirpation. Stable course
of the disease was established since second operation until now.
Review of the literature concerning incidence and differential diagnosis
of post-radiation brain tumors was done. The prior cranial irradiation
as a possible risk factor for meningioma development was discussed.
Key words: basal cell carcinoma, radiation therapy, recurrent
meningioma, differential diagnosis
AN ORIGINAL PC PROGRAM FOR REGISTRATION, STORAGE AND ANALYSIS
OF DATABASE ABOUT PATIENTS WITH BRAIN TUMORS
A. Kaprelyan,
M. Georgiev*
Department of Neurology, Medical University - Varna, Bulgaria
*Technical University - Tampere, Finland
ABSTRACT: An original PC program developed and designed
for registration, storage and analysis of database about patients with
primary and secondary brain tumors was presented. The so called “Program
for operation of hospital charts of patients with brain neoplasms” was
based on information from routine and modern clinical, instrumental and
immunological methods for diagnosis, monitoring and prognosis. It was
designed as a PC model of a chart for registration with unique mode of
operation of patients’ entry characteristics, forming of their results
into database and opportunity for calculation and prognosis of exit parameters
such as probable survival rate and etc.
The main task of the program was to provide a useful data for the investigated
patients with brain tumors. It was achieved by application of a real chart
for collection and extraction of filtrated information about clinical
cases with similar characteristics. The entire operation of the database
was set up by Microsoft OLEDBDC driver 2003. The integral functionality
concerning screen imaging, entry, correction, erasure, search and calculation
of data was realized with program language Microsoft Visual C# 2003. The
presented program was highly informative and easily available for routine
administration in the neuro-oncological practice.
Key words: brain tumor, information system, PC program,
database, hospital chart
OLFACTORY NEUROBLASTOMA: CLINICAL PRESENTATION
AND MANAGEMENT
B. Stamenov
, Pl. Tzvetanov, V. Simeonova, Nikolova M.*, Ph. Philipov
Department of Neurology and Neurosurgery
* Department of Pathology
University Hospital of Pleven, Bulgaria
ABSTRACT: Olfactory neuroblastoma (ONB) is a rare tumor.
There is greater agreement for separating “neuroblastoma-like” forms from
tumors arising in the same location and manifest morphological and immunohistochemical
characteristics of neuroendocrine carcinoma.
The aim of present study is to describe the typical clinical, histological
and immunohistochemical findings of ONB in 58-year old woman which help
us to exclude non-Hodgkin’s lymphoma and endocrine carcinoma.
Key words: olfactory neuroblastoma, neuroblastoma like
forms, neuroendocrine carcinoma
WORKING TIME ORGANIZATION AND MYOCARDIAL INFARCTION
(First communication)
T. Dimitrova,
A. Penev*, I. Zlatarov
Department of Hygiene and Disaster Medicine, Prof. Paraskev Stoyanov
Medical University of Varna
*Intensive Clinic of Cardiology, St. Marina University Hospital of Varna
SUMMARY: The purpose of the investigation is to analyze
by means of hospital based case-control study the influence of working
time duration in acute myocardial infarction (AMI) patients. The results
demonstrate that males prevail among AMI patients in any age groups except
for the group over 76 years while at the working age, 60,9% of the subjects
examined are mainly physical workers (Mann-Whitney U 1633, As. Sig. 0,02).
There is a statistically significant overtime workday and workweek immediately
prior to hospitalization as well as a systemic pattern concerning the
length of service in the group with AMI in comparison with that in the
control group. Wrong working time organization with overtime workday and
workweek is a correctable risk factor for a healthy status.
Key words: acute myocardial infarction, overtime work,
case-control study
< back >
EDITORIAL
BOARD: Address: Publishers: Journal of IMAB |
|