Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2013, vol. 19, book 4
Subject Collection: Oral and Dental Medicine
Pages: 430-434
DOI: 10.5272/jimab.2013194.430
Published online: 16 December 2013

J of IMAB 2013 Jul-Dec;19(4):430-434
Violeta Dogandzhiyska1Corresponding Autor, Raina Gergova2, Slavcho Dimitrov3, Maya Doychinova3.
1) Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria
2) Department of Microbiology, Medical University, Sofia, Bulgaria
3) Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, Varna, Bulgaria.

Introduction: Photodynamic therapy (PDT) is an innovative method, supporting routine treatment methods and accelerating the healing process. It is a method for treatment of pathogens and tumor cells with photoactive dye, in the presence of oxygen. PDT is minimally invasive and high- selective method against specific cells and is equally effective against sensitive and resistant to antibiotics microorganisms.
Aim: The purpose of this study was to determine the antimicrobial activity of the PDT (Fotosan-Agent High) against microorganisms isolated from deep carious lesions.
Materials and methods:  For the purpose of this study were selected 20 patients ( n=20) with primary, deep carious lesions on permanent teeth with complete root development  All prepared cavities were class I and class II, and the treatment method was indirect pulp capping. Cavities were prepared with high and low-speed handpieces and water- air cooling. Two microbiological samples were taken of each cavity:
Group 1 - Microbiological samples after cavity treatment with Aqua destillata (n=20)
Group 2 - Microbiological samples after cavity treatment with PDT (Photosensitizer Fotosan (Agent high) and irradiation with a diode laser, λ = 632 nm, for 60 seconds)(n=20)
The samples were placed in eppendorf tube with standard transport medium   and transported to the microbiological laboratory for determination of microbial number and identification of the isolated microorganisms.
The statistical analysis was made with non- parametric test of` McNemar.
Results:  In 82.35% of cases (14 of 17 cases) after treatment bacteriаl infection was totally eliminated. In 17.65% (3 of 17 cases) was determined reduction of bacteria count from 100 to 1000 times.
The statistical analysis with non- parametric test of` McNemar presented significantly higher proportion of cases with bacterial elimination after photodynamic therapy ( p < 0.001).
Conclusion:  After photodynamic desinfection were determined considerable elimination of microbial  infection and  reduction of  bacterial variety and bacterial number. On the ground of these results it can be concluded, that photodynamic therapy is an effective method of treatment and removal of microorganisms from deep carious lesions and support the healing processes in the dentin and dental pulp.

Key words: Photodynamic therapy; antimicrobial activity; deep carious lesions; indirect pulp capping;

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Please cite this article as:
Dogandzhiyska V, Gergova R, Dimitrov S, Doychinova M. Antimicrobial activity of Photodynamic therapy against microorganisms isolated from deep carious lesions. J of IMAB. 2013 Jul-Dec;19(4):430-434. doi: 10.5272/jimab.2013194.430.

Correspondence to: Dr.  Violeta D. Dogandzhiyska, Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University Sofia; 1, St George Sofiisky str., 1431 Sofia, Bulgaria;

1. Gergova R. Microbiological, immunological and molecular genetic studies of isolates Moraxella catarrhalis from patients with respiratory infections. A dissertation for the award of the degree “Doctor” (PhD). 2008. [in Bulgarian]
2. Gergova R., I.  Mitov, P.  Minchev..  Criteria for the isolation and evaluation of the clinical relevance of Moraxella (Branhamella) catarrhalis in samples from the respiratory tract. Modern medicine. 2003; LIV ( 5 ):3-10. [in Bulgarian]
Gergova R, I. Mitov, T.  Kantardzhiev . A selective medium for isolation of Moraxella (Branhamella) catarrhalis and Haemophilus spp. in samples from the respiratory tract. Infectology. 2002, XXXIX ( 2 ): 34-37. [in Bulgarian]
Mitov I., R. Gergova, M. Tiholova. Microflora of the oral cavity and the upper respiratory tract. Modern medicine. 1999; 5-6:3-10. [in Bulgarian]
5. Convissar RA. Lasers in general dentistry. Oral Maxillofac Surg Clin North Am. 2004 May;16(2):165-179. [PubMed]
6. George S, Kishen A. Influence of Photosensitizer Solvent on the Mechanisms of Photoactivated Killing of Enterococcus faecalis. Photochem Photobiol. 2008 May-Jun;84(3):734–740. [PubMed] [CrossRef]
7. Godoy BM, Arana-Chavez VE, Núñez SC, Ribeiro MS. Effects of low-power red laser on dentine–pulp interface after cavity preparation. An ultrastructural study. Arch Oral Biol. 2007 Sep;52(9):899- 903. [PubMed] [CrossRef]
 8. Maltz M, de Oliveira EF, Fontanella V, Bianchi R. A clinical, microbiologie, and radiographie study of deep caries lesions after incomplete caries removal. Quintessence Int. 2002 Feb;33(2):151-159. [PubMed]
9. Mang TS. Lasers and light sources for PDT: past, present and future. Photodiagnosis and Photodynamic Therapy. 2004; 1:43-48.
10. Murraya PE, Hafezb AA, Windsora LJ, Smithc AJ, Coxd CF. Comparison of pulp responses following restoration of exposed and non-exposed cavities. J Dent. 2002 Jul-Aug;30(5-6):213–222. [PubMed]
11. Мyers TD, Sulewski JG. Evaluating dental lasers: what the clinician should know. Dent Clin North Am. 2004 Oct;48(4):1127-44. [PubMed] [CrossRef]
 12. Nagata JY, Hioka N, Kimura E, Batistela VR, Terada RS, Graciano AX, et al. Antibacterial photodynamic therapy for dental caries: Evaluation of the photosensitizers used and light source properties. Photodiagnosis Photodyn Ther. 2012 Jun;9(2):1-10. [PubMed] [CrossRef]
13. Di Poto A, Sbarra MS, Provenza G, Visai L, Speziale P. The effect of photodynamic treatment combined with antibiotic action or host defence mechanisms on Staphylococcus aureus biofilms. Biomaterials. 2009; 30:3158–3166.
14. Santini A. The Management of the Deep Carious Lesion and Maintenance of Pulp Vitality (II). Restorative Dentistry. 1980 Apr: p. 6.
 15. Smith EG., GA. Spatafora. Gene Regulation in S. mutans: Complex Control in a Complex Environment. J Dent Res. 2012; 91(2):133-141.
16. Todea C, Kerezsi C, Balabuc C, Calniceanu M, Filip L. Pulp Capping – from Conventional to Laser-assisted Therapy (I). J Oral Laser Application. 2008; 8: 71-82.
17. Todea C, Kerezsi C, Balabuc C, Calniceanu M, Filip L. Pulp Capping – from Conventional to Laser-assisted Therapy (II). J Oral Laser Application. 2008; 8: 147-155.].

Received: 25 October 2013
Published online: 16 December 2013

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