Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2014, vol. 20, issue 6
Subject Collection: Medicine
Pages: 542-546
DOI: 10.5272/jimab.2014206.542
Published online: 04 December 2014

J of IMAB 2014 Oct-Dec;20(6):542-546
Ilina Micheva1 Corresponding Autor, Liana Gercheva1, Panos Ziros2, Nicholas Zoumbos2.
1) Hematology Division, Department of Internal Medicine, Medical University, Varna, Bulgaria
2) Hematology Division, Department of Internal Medicine, Patras University Medical School, Patras, Greece.

We have previously demonstrated that in patients with myelodysplastic syndrome (MDS) monocyte-derived DCs (MoDC) exhibit some phenotypic and functional abnormalities. However, the mechanisms underlying the defective response have not yet been clarified.
Aim: In the present study three different signaling pathways, ERK, p38K and NF-κB, were studied on MoDC from patients with MDS.
Materials and methods: 7 patients with MDS and 5 healthy controls were included in the study. MACS separated CD14 cells were cultured for DC generation and further stimulated with TNF-α and LPS. A migration assay and ELISA were performed for the analysis of migration and IL-12p70 secretion. Western blot analysis was used for the detection of the phosphorylated forms of ERK and p38K. NF-κB binding activity was evaluated by electrophoretic mobility shift assay (EMSA).
Results: In 6/7 patients the NF-κB binding activity in TNF-α and LPS stimulated MoDCs was lower compared to controls. This was accompanied by lower migratory capacity and IL-12p70 secretion. TNF-α and LPS induced phosphorylation of p38 and ERK at similar levels in control MoDC, whereas in MDS MoDC the pattern was heterogeneous with predominant activation of ERK over p38K.
Conclusion: Our results provide strong evidence that defective signaling through NF-κB and MAPK underlies the functional abnormalities of MoDC in patients with MDS.

Key words: Myelodysplastic syndromes; dendritic cells; ERK, p38, NF-κB,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Micheva I, Gercheva L, Ziros P, Zoumbos N. ERK, p38 and NF-kappaB signaling in monocyte derived dendritic cells in patients with myelodysplastic syndrome. J of IMAB. 2014 Oct-Dec;20(6):542-546.

Correspondence to: Dr. Ilina Micheva, Hematology Division, Department of Internal Medicine, Medical University; 1, Hristo Smirnenski str., 9010 Varna, Bulgaria; E-mail:

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Received: 28 September 2014
Published online: 04 December 2014

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