Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2015, vol. 21, issue 4
Subject Area: Medicine - Gastroenterology
Pages: 969-973
DOI: 10.5272/jimab.2015214.969
Published online: 30 November 2015

J of IMAB 2015 Oct-Dec;21(4):969-973
ROLE AND IMPORTANCE OF MICROBIOLOGY TESTING IN THE DIAGNOSIS AND TREATMENT OF HELICOBACTER PYLORI INFECTION.
Miglena Stamboliyska1Corresponding Autor, Violina Kaludova2, Milko Mirchev1, Iskren Kotzev1, Rosen Madjov3, Krasimir Metodiev2
1) Clinic of Gastroenterology, University Hospital ”St. Marina”, Medical University - Varna, Bulgaria
2) Department of preclinical and clinical sciences, Faculty of Pharmacy, Medical University - Varna, Bulgaria
3) Second Department of Abdominal Surgery, University Hospital ”St. Marina”, Medical University - Varna, Bulgaria.

ABSTRACT:
Background: Unsuccessful treatment of H. pylori (HP) infection is constantly rising. Antibiotic susceptibility testing and selection of individualized therapy are expected to improve eradication of HP.
Aim: To assess the diagnostic accuracy of microbiology testing for HP and to assess the effectiveness of culture-guided susceptibility tested antibiotic therapy.
Materials and methods: A total of 62 HP infected patients, 35 with unsuccessful eradication and 27 treatment naive were subjected to culture testing and 3 endoscopy-based methods. In isolated НР colonies strain susceptibility and resistance were determined by disc-diffusion method. A triple therapy in the naïve group and quadruple in the unsuccessfully treated group was applied. The effectiveness of eradication was evaluated after 2 months by stool antigen test and/or urea breath test. Fisher’s exact test for comparison of therapeutic efficacy was used.
Results: НР colonies were isolated in 13 patients - 9 naïve and 4 unsuccessfully treated. Higher sensibility (100%) was shown towards UBT and high specificity between 95% - 100% compared to other methods. Its accuracy was 91.9%. In all patients with proven resistance culture-guided eradication was successful.
Conclusions: Microbiology and antibiotic susceptibility testing allows optimal diagnosis and therapy of HP infection.

Key words: Helicobacter pylori, eradication, microbiology testing, PCR, antibiotic susceptibility,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Stamboliyska M, Kaludova V, Mirchev M, Kotzev I, Madjov R, Metodiev K. Role and Importance of Microbiology testing in the Diagnosis and Treatment of Helicobacter pylori Infection. J of IMAB. 2015 Oct-Dec;21(4):969-973. doi: http://dx.doi.org/10.5272/jimab.2015214.969

Correspondence to: Assoc. prof. Miglena Stamboliyska, MD, PhD, Clinic of Gastroenterology, UMHAT St. Marina; 1, Hristo Smirnenski blvd., 9010 Varna, Bulgaria; E-mail: m.stamboliyska@abv.bg

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Received: 24 June 2015
Published online: 30 November 2015

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