Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X
Issue: 2016, vol. 22, issue 4
Subject Area: Medicine
Published online: 21 December 2016
J of IMAB 2016 Oct-Dec;22(4):1407-1410
VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE
Lidija Kareva , Kristina Mironska, Katerina Stavrik.
Clinic for children’s disease, Department of immunology, Faculty of Medicine - Skopje, Macedonia.
Background: The effects of demographic factors on a wide range of immunological variables demonstrate the importance of having normative data representative of particular patient population. There was no lymphocyte subpopulation data for Macedonian children and the purpose of this study was to establish such a data.
Subject and methods: The study population consists of 87 healthy children. Subjects were grouped into four age categories as follows: group 1 age range 5d-10d (n=15); group 2 age range 1 mo-1 yr (n=18); group 3 age 1yr-2 yr(n=20) and group 4 age 2yr-5 yr(n=34
Monoclonal antibodies labeled with fluorochromes and immunofluorescent microscopy were used to detect cells bearing specific cell markers.
Results: The mean lymphocyte cell count gradually fell with increasing age from 6,65x10^9/l in group 1, to 5,67x10^9/l in group 2, 4,55x10^9/l in group 3, and to 4,14x10^9/l in group 4. Absolute values of CD3, CD4 and CD20 positive cells decreased gradually with age. Significant differences in mean absolute values were observed for absolute lymphocyte counts between groups 1/2 and 1/4 (P<0,01) and groups 2/4(P<0,05); for CD3 positive lymphocytes between groups 1/3(P<0,05) and 1/4(P<0,01); for CD4 positive lymphocytes between groups 1/3 (P<0,05) and 1/4(P<0,01) and for CD20 positive lymphocytes between groups 1/3 and 3/4(P<0,05) and groups 1/4 (P<0,01). Significant difference for CD4/CD8 ratio and for percentage values of different lymphocyte subpopulations between the different age groups was not found.
Conclusion: This data may serve as a reference range for studies of Macedonian pediatric subjects.
Key words: lymphocyte subpopulation, children,
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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Kareva L, Mironska K, Stavrik K. Values of lymphocyte subpopulations in healthy Macedonian children under the age of five. J of IMAB. 2016 Oct-Dec;22(4):1407-1410. DOI: 10.5272/jimab.2016224.1407.
Correspondence to: Dr. Lidija Kareva, Slavej Planina 8, 1000 Skopje, Macedonia; E-mail: firstname.lastname@example.org
1. Moraes-Pinto MI, Ono E, Santos-Valente EC, Almeida LC, Andrade PR, Dinelli MI, et al. Lymphocyte subsets in human immunodeficiency virus-unexposed Brazilian individuals from birth to adulthood. Mem Inst Oswaldo Cruz. 2014 Dec;109(8):989-98. [PubMed] [CrossRef]
2. Valiathan R, Ashman M, Asthana D. Effects of Ageing on the Immune System: Infants to Elderly. Scand J Immunol. 2016 Apr;83(4):255-66. [PubMed] [CrossRef].
3. García-Dabrio MC, Pujol-Moix N, Martinez-Perez A, Fontcuberta J, Souto JC, Soria JM, et al. Influence of age, gender and lifestyle in lymphocyte subsets: report from the Spanish Gait-2 Study. Acta Haematol. 2012;127(4):244-9. [PubMed] [CrossRef]
4. Heldrup J, Kalm O, Prellner K. Blood T and B lymphocyte subpopulations in healthy infants and children. Acta Pediatr. 1992 Feb;81(2):125-32. [PubMed]
5. Lawrie D, Payne H, Nieuwoudt M, Glencross DK.Observed full blood count and lymphocyte subset values in a cohort of clinically healthy South African children from a semi-informal settlement in Cape Town. S Afr Med J. 2015 Sep 21;105(7):589-95. [PubMed] [CrossRef].
6. Jia L, Li J, Zhang Y, Shi Y, Yuan E, Liu J, et al. Age- and sex-related reference intervals of lymphocyte subsets in healthy ethnic Han Chinese children. Cytometry A. 2015 Dec;87(12):1116-26. [PubMed] [CrossRef].
7. Atanasova V, Mihaylova A, Lukanov T, Atanasova M, Nikolov A, Naumova E. Evaluation of lymphocyte subpopulations in cord blood of Bulgarian newborns. Clin Lab. 2014; 60(11):1887-93. [PubMed]
8. Rabian-Herzog C, Lesage S, Gluckman E. Characterization of lymphocyte subpopulations in cord blood. Bone Marrow Transplant. 1992; 9 Suppl 1: 64-7. [PubMed]
9. Raes M, Alliet P, Gillis P, Zimmermann A, Kortleven J, Magerman K, et al. Lymphocyte subpopulations in healthy newborn infants: comparison of cord blood values with values five days after birth. J Pediatr. 1993 Sep;123(3):465-7. [PubMed]
10. Ozkan H, Olgun N, Sasmaz E, Abacioglu H, Okuyan M, Cevik N. Nutrition, immunity and infections: T lymphocyte subpopulations in protein--energy malnutrition. J Trop Pediatr. 1993 Aug;39(4):257-60. [PubMed]
11. Lisse IM, Aaby P, Whittle H, Jensen H, Engelmann M, Christensen LB. T-lymphocyte subsets in West African children: impact of age, sex, and season. J Pediatr. 1997 Jan;130(1):77-85. [PubMed]
12. Ulijaszek SJ. Immunology and growth faltering of Anga children, Papua New Guinea: preliminary work. Am J Phys Anthropol. 1998 Aug;106(4):515-20. [PubMed]
13. Comans-Bitter WM, de Groot R, van den Beemd R, Neijens HJ, Hop WC, Groeneveld K, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatr. 1997 Mar;130(3):388-93. [PubMed]
14. Lin SC, Chou CC, Tsai MJ, Wu KH, Huang MT, Wang LH, et al. Age-related changes in blood lymphocyte subsets of Chinese children. Pediatr Allergy Immunol. 1998 Nov;9(4):215-20. [PubMed]
15. Stern DA, Hicks MJ, Martinez FD, Holberg CJ, Wright AL, Pinnas J, et al. Lymphocyte subpopulation number and function in infancy. Dev Immunol. 1992;2(3):175-9. [PubMed]
16. Shahabuddin S, Al-Ayed I, Gad El-Rab MO, Qureshi MI. Age-related changes in blood lymphocyte subsets of Saudi Arabian healthy children. Clin Diagn Lab Immunol. 1998 Sep;5(5):632-5. [PubMed]
17. Osugi Y, Hara J, Kurahashi H, Sakata N, Inoue M, Yumura-Yagi K, et al. Age-related changes in surface antigens on peripheral lymphocytes of healthy children. Clin Exp Immunol. 1995 Jun;100(3):543-8. [PubMed].
Received: 13 October 2016
Published online: 21 December 2016
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