head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2017, vol. 23, issue 4
Subject Area: Medicine
DOI: 10.5272/jimab.2017234.1747
Published online: 16 November 2017

Original article

J of IMAB 2017 Oct-Dec;23(4):1747-1751
Veselina Panayotova1 ORCID logo Corresponding Autoremail, Albena Merzdhanova1, Diana A. Dobreva1, Magdalen Zlatanov2, Lubomir Makedonski1,
1) Department of Chemistry, Faculty of Pharmacy, Medical University of Varna, Bulgaria,
2) Department of Chemical Technology, Faculty of Chemistry, Plovdiv University, Plovdiv, Bulgaria.

Background: Bulgarian Black Sea coast is rich in algae, regarding biomass and algal biodiversity. The red algae Gelidium crinale (Rhodophyta) and brown algae Cystoseira barbata (Phaeophytes) are among the most abundant species along the Bulgarian Black Sea shore. Yet information about their lipid composition is limited.
Purpose: Present study was conducted to investigate biologically active substances in two underexplored seaweed lipids. Total lipids, total phospholipids, fat soluble vitamins and carotenoids were analysed. In addition, the specific distribution of fatty acids group among the total lipids and total phospholipids were elucidated.
Material/Methods: The saponifiable lipid fraction was derivatized into fatty acid methyl esters (FAMEs) and analysed by gas chromatography–mass spectrometry (GC-MS) to identify and quantify the fatty acids. The fat soluble non-saponifiable lipids were identified by high-pressure liquid chromatography coupled with UV/Vis and fluorescence detectors (HPLC-UV-FL).
Results: Results showed that Rhodophyta and Phaeophytes have high concentrations of polyunsaturated fatty acids (PUFA), particularly from the n-3 series, thereby being a good source of these compounds. They presented a “healthy” n-6/n-3 ratio. Both seaweed species showed considerably high amounts of α-tocopherol, β-carotene and astaxanthin. Conclusions: The study reveals that lipids from Black Sea algae have a high potential as natural sources of biologically active ingredients. They are balanced source of fatty acids and contained beneficial antioxidants, such as α-tocopherol, β-carotene and astaxanthin.

Keywords: Cystoseira barbata, Gelidium crinale, phospholipids, fatty acids or poly unsaturated fatty acids (PUFA), tocopherols, carotenoids, astaxanthin,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Panayotova V, Merzdhanova A, Dobreva DA, Zlatanov M, Makedonski L. Lipids of Black Sea algae: unveiling their potential for pharmaceutical and cosmetic applications. J of IMAB. 2017 Oct-Dec;23(4):1747-1751. DOI: 10.5272/jimab.2017234.1747

Corresponding AutorCorrespondence to: Veselina Panayotova, Department of Chemistry, Faculty of Pharmacy, Medical University of Varna; 84 “Tzar Osvoboditel” blvd., Varna, Bulgaria; E-mail: veselina.ivanova@hotmail.com

1. Pereira H, Barreira L, Figueiredo F, Custódio L, Vizetto-Duarte C, Polo C, et al. Polyunsaturated Fatty Acids of Marine Macroalgae: Potential for Nutritional and Pharmaceutical Applications. Mar Drugs. 2012; 10(9):1920-35. [CrossRef]
2. Škrovánková S. Chapter 28 - Seaweed vitamins as nutraceuticals. Adv Food Nutr Res. 2011; 64:357-69. [PubMed] [CrossRef]
3. Patil V, Källqvist T, Olsen E, Vogt G, Gislerod H. Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquacult Int. 2007 Feb; 15(1):1-9. [CrossRef]
4. Bligh Е, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol, 1959 Aug;37(8):913-7. [PubMed] [CrossRef]
5. BDS EN ISO 12966-2:2017. Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids. p.27. [in Bulgarian & English] [Internet]
6. BDS EN ISO 12966-4:2015. Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography. p.30. [in Bulgarian & English]  [Internet]
7. Dobreva DA, Panayotova V, Stancheva R, Stancheva M. Simultaneous HPLC determination of fat soluble vitamins, carotenoids and cholesterol in seaweed and mussel tissue. Bulg Chem Commun. 2017; 49(G):112-17.
8. Kumari P, Kumar M, Gupta V, Reddy CRK, Jha B. Tropical marine macroalgae as potential sources of nutritionally important PUFAs. Food Chem. 2010 Jun;120(3):749-57. [CrossRef]
9. Boulom S, Robertson J, Hamid N, Ma Q, Lu J. Seasonal changes in lipid, fatty acid, α-tocopherol and phytosterol contents of seaweed, Undaria pinnatifida, in the Marlborough Sounds, New Zealand. Food chem. 2014 Oct 15;161:261-69. [PubMed] [CrossRef]
10. Kumari P, Kumar M, Reddy CRK, Jha B. 3-Algal lipids, fatty acids and sterols, In; Functional Ingredients from Algae for Foods and Nutraceuticals. Part I: Structure and occurrence of the major algal components. Edited by:H. Dominguez. Woodhead Publishing, 2013; p.87-134; [CrossRef]
11. Kendel M, Wielgosz-Collin G, Bertrand S, Roussakis C, Bourgougnon N, Bedoux G. Lipid composition, fatty acids and sterols in the seaweeds Ulva armoricana, and Solieria chordalis from Brittany (France): An analysis from nutritional, chemotaxonomic, and antiproliferative activity perspectives. Mar drugs. 2015; 13(9): 5606-28. [PubMed] [CrossRef]
12. World Health Organization. Population nutrient intake goals for preventing diet-related chronic diseases. In Diet, Nutrition and the Prevention of Chronic Diseases; Report of a Joint WHO/FAO Expert Consult 2003; Geneva, Switzerland,
13. Department of Health 1994. Nutritional aspects of cardiovascular disease: Health and social subjects. HMSO, London.
14. Santos SA, Vilela C, Freire CS, Abreu MH, Rocha S, Silvestre AJ. Chlorophyta and Rhodophyta macroalgae: A source of health promoting phytochemicals. Food chem. 2015 Sep;183:122-28. [PubMed] [CrossRef]
15. Ortiz J, Romero N, Robert P, Araya J, Lopez-Hernández J, Bozzo C, et al. Dietary fibre, amino acid, fatty acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea antarctica. Food chem. 2006; 99(1):98-104. [CrossRef]
16. Norziah MH, Ching CY. Nutritional composition of edible seaweed Gracilaria changgi. Food chem. 2000 Jan;68(1):69-76. [CrossRef]
17. Higuera-Ciapara I, Felix-Valenzuela L, Goycoolea FM. Astaxanthin: a review of its chemistry and applications. Crit Rev Food Sci Nutr. 2006; 46(2):185-96. [PubMed] [CrossRef].

Received: 20 June 2017
Published online: 16 November 2017

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