head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2017, vol. 23, issue 4
Subject Area: Medicine
DOI: 10.5272/jimab.2017234.1765
Published online: 28 November 2017

Original article

J of IMAB 2017 Oct-Dec;23(4):1765-1768
Georgi Bonchev1 ORCID logo Corresponding Autoremail, Snezha Zlateva1,2, Petko Marinov1,2, Marieta Yovcheva1, Ivaylo Vazharov3,
1) Clinic for Intensive Treatment of Acute Intoxications and Toxicoallergies, Naval Hospital – Varna, Military Medical Academy, Bulgaria
2) Department of Pharmacology, Toxicology and Pharmacotherapy, Faculty of Pharmacy, Medical University – Varna, Bulgaria
3) Clinic of Internal Diseases, Naval Hospital – Varna, Military Medical Academy, Bulgaria.

Purpose: To develop and implement a UHPLC method for quantitative determination of sertraline in biological samples – mostly human blood and urine.
Material&Methods: Blood and urine samples available from Laboratory of analytical toxicology and Clinic for intensive treatment of acute intoxications and toxicoallergies were used during method validation and case monitoring. Analytical identification of sertraline and/or metabolites was done by GC-MS. Gas chromatography coupled with flame ionization detection was used for alcohols/volatiles screening of clinical samples. Ultra high-performance liquid chromatography system in tandem with diode-array detector has been used as the main quantitative instrument.
Results: After critical consideration of available reference data a carefully set of experimental conditions for sertraline extraction and UHPLC determination were adopted and optimized. Preliminary liquid-phase sample purification was applied. Zorbax Extend-C18 column (150 x 4.6 mm, 5 µm) was used under isocratic conditions with phosphate buffer (pH 2.7; 10 mM) containing 1.5 ml L–1 triethylamine – acetonitrile (65:35, v/v) at 20oC, at the flow-rate of 1.0 mL/min and UV detection at 220 nm. This method was validated for the determination of sertraline in human plasma/serum samples (70% recovery).
Conclusions: A simple yet sensitive and reliable method for sertraline determination was introduced. Linearity over 20-1000 ng mL–1 range was shown; LOQ was 20 ng mL–1. The method was clinically applied for monitoring the blood sertraline levels during a course of detoxication of a female patient.

Keywords: sertraline, Zoloft, HPLC determination, drug monitoring,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Bonchev G, Zlateva S, Marinov P, Yovcheva M, Vazharov I. A UHPLC method for sertraline determination. J of IMAB. 2017 Oct-Dec;23(4):1765-1768. DOI: 10.5272/jimab.2017234.1765

Corresponding AutorCorrespondence to: Georgi Bonchev, PhD, Head, Laboratory of Analytical Toxicology, Military Medical Academy, Naval Hospital – Varna; 3, Hristo Smirnenski Blvd., 9010 Varna, Bulgaria; E-mail: toxilab.varna@abv.bg

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Received: 12 June 2017
Published online: 28 November 2017

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