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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2020, vol. 26, issue4
Subject Area: Medicine
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DOI: 10.5272/jimab.2020264.3398
Published online: 29 October 2020
Original article
J of IMAB. 2020 Oct-Dec;26(4):3398-3402
COMPARISON OF ANTIMICROBIAL EFFICACY OF THREE TYPES OF MOUTHWASH, CONTAINING CHLORHEXIDINE-CHLORBUTANOL, ALCOHOL-ESSENTIAL OILS AND PROPOLIS-MENTHA OIL COMBINATIONS
Neli Ermenlieva1
, Emilia Georgieva2, Minko Milev3, Nadya Agova4,
1) Department of Microbiology and Virology, Faculty of Medicine, Medical University - Varna, Bulgaria
2) TRS "Medical laboratory assistant", Medical College, Medical University - Varna, Bulgaria
3) TRS "Dental technician", Medical College, Medical University - Varna, Bulgaria
4) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University - Varna, Bulgaria.
ABSTRACT:
Mouthwash is an antiseptic solution intended to reduce the microbial load in the oral cavity, although other mouthwash might be given for other reasons such as for their analgesic, anti-inflammatory or anti-fungal action.
A study was carried out to compare the antibacterial and antifungal properties of three mouthrinse preparations - both containing commercial used antiseptic combinations (chlorhexidine 0,100% + chlorbutanol 0,500% and alcohol 21,60% + essential oils) and one with natural active ingredients (propolis 2,00% + mentha oil 0, 042%).
The antibacterial and antifungal activity of three types of mouthwash were tested on three microbial strains - Staphylococcus aureus, Escherichia coli and Candida albicans by two alternatives of agar diffusion tests – “cup plate” technique and disc-diffusion test.
The results showed the highest antimicrobial activity of the chlorhexidine-chlorbutanol combination. Mouthwash containing propolis with mint oil demonstrated activity only against S. aureus strain.
It turned out that testing the antimicrobial activity of mouthwash with alcohol with essential oils with the agar diffusion method is not representative. For 24 hours with this mouthwash, no inhibition zones were observed in none of the strains at any concentration. Our assumption is that alcoholic solutions are exuding and bacteria starts to grow.
Keywords: mouthwash, antimicrobial activity, chlorhexidine, chlorbutanol, alcohol, propolis, essential oils,
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Please cite this article as: Ermenlieva N, Georgieva E, Milev M, Agova N. Comparison of antimicrobial efficacy of three types of mouthwash, containing chlorhexidine-chlorbutanol, alcohol-essential oils and propolis-mentha oil combinations. J of IMAB. 2020 Oct-Dec;26(4):3398-3402.
DOI: 10.5272/jimab.2020264.3398
Correspondence to: Neli Mitkova Ermenlieva, Department of Microbiology and Virology, Faculty of Medicine, Medical University Varna; 3. Bregalniza Str.. 9002 Varna, Bulgaria; E-mail: n.ermenlieva@abv.bg
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Received: 05 November 2019
Published online: 29 October 2020