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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2020, vol. 26, issue4
Subject Area: Medicine
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DOI: 10.5272/jimab.2020264.3458
Published online: 20 November 2020
Original articles
J of IMAB. 2020 Oct-Dec;26(4):3458-3462
DETECTION OF SPECIFIC SURFACTANT PROTEINS IN GASTRIC ASPIRATES FROM PREMATURELY BORN CHILDREN AFTER CORTICOSTEROID THERAPY
Vishnya Stoyanova1
, Asya Tsanova1, Albena Jordanova1, Neli Jekova2, Detelina Stoichkova3, Krasimira Ivanova4, Emilia Hristova5,
1) Department of Chemistry and Biochemistry, Physiology and Pathophysiology, Faculty of Medicine, St. Kliment Ohridsky University of Sofia, Bulgaria
2) Department of Obstetrics and Gynaecology, Medical University of Sofia, Bulgaria
3) Multiprofile Hospital for Active Treatment Nadezhda, Sofia, Bulgaria
4) 1st Maternity Hospital St. Sofia, Sofia, Bulgaria
5) Department of Health care, Faculty of Public Health, Medical University of Sofia, Bulgaria.
ABSTRACT:
Purpose: Alveolar surfactant (AS) components, including the specific surfactant proteins (SPs) SP-A, SP-B, SP-C, and SP-D, provides stability during the dynamic process of inhalation/exhalation. In prematurely born children different respiratory pathologies due to surfactant components deficiency, like Neonatal Respiratory Distress Syndrome, can be observed. Administration of corticosteroids to pregnant women at risk of preterm birth is an established intervention in clinical practice. In this study, we analyzed gastric aspirates (GAs), as alternative samples of tracheal aspirates and amniotic fluids for AS maturity determination. Samples were taken from prematurely born babies after antenatal corticosteroid therapy (CST) of pregnant women and were analyzed for the presence of specific surfactant proteins.
Materials and Methods: Clinical samples of gastric aspirates were collected in the first minutes after delivery by using of a nasogastric tube and were analyzed by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot assays for detection of SP-A, SP-B, and SP-C.
Results: Our results showed the expression of different isoforms of each specific surfactant protein (SP) in all GA samples, depending on the stage of maturation.
Conclusions: Our results showed that CST plays a role in AS components production and maturation. Moreover, GA can be considered as an adequate sample for assessment of surfactant maturity at birth.
Keywords: surfactant proteins, prematurely born children, corticosteroid therapy, SDS-PAGE, Western blot,
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Please cite this article as: Stoyanova V, Tsanova A, Jordanova A, Jekova N, Stoichkova D, Ivanova K, Hristova E. Detection of specific surfactant proteins in gastric aspirates from prematurely born children after corticosteroid therapy. J of IMAB. 2020 Oct-Dec;26(4):3458-3462.
DOI: 10.5272/jimab.2020264.3458
Correspondence to: Vishnya Stoyanova, Department Chemistry and Biochemistry, Physiology and Pathophysiology, Faculty of Medicine, St. Kliment Ohridsky University of Sofia; 1, Kozyak Str., 1407, Sofia, Bulgaria; E-mail: vishnya_stoyanova@abv.bg
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Received: 03 October 2019
Published online: 20 November 2020