back to 2011, vol. 17, b. 1

Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2011, vol. 17, book 1
Subject Collection: Medicine
Page: 127-129
DOI: 10.5272/jimab.2011171.127
Online date: May, 2011

J of IMAB 2011; 17(1):127-129
Tamara Paypanova1, Tatyana Hristova2, Rumyana Cherkezova2
1) Institute of Molecular Biology, Bulgarian Academy of Sciences - Sofia, Bulgaria;
2) Department of Medical and Biological Sciences, Faculty of Dental Medicine, Medical University - Varna, Bulgaria

The advantages of the solid-phase peptide synthesis were used. The peptide chain was built on specially processed insoluble polymer resin: Wang- and Rink Amide MBHA-resin. Three new fragment oligopeptides were prepared by activation and condensation of Fmoc-protected amino acids [which were subject of our previous work]. One of the important advantages of Fmoc-strategy is achieved: no continuous unblocking of the protection group is effected and the formed peptide bonds do not break.

Key words: solid-phase peptide synthesis, oligopeptide fragments.

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1. Cammish L. E., Kates S. A., “Fmoc-SPPS: a practial aprproch”, W. C. Chan, P.D. White (Eds.), University Press, Oxford; 2000
2. Smith A. J. et al, in: “Techniques in protein chemistry III”, R. Angelotti (Ed.), Academic Press; Orlando; 1992; 219
3. Fields G.B. Noble R.L. Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. Int. J. Peptide Protein Res. 1990 Mar;35(3):161-214 doi: 10.1111/j.1399-3011.1990.tb00939.x [CrossRef] [PubMed]
4. Gordon K., Balasubramancan S.: Solid phase synthesis - Designer Linkers for Combinatorial Chemistry.  J. Chem. Tech. Biotech.: 1999 September; 74 (9): 835-851
5. Lloyd-Williams P., Albericio F., Giralt E.: Chemical approaches to the Synthesis of peptides and proteins. University of Barselona, CRC Pres LLC: 1997
6. Albericio F., Chinchilla R., Dodsworth D. J., Najera C.: New trends in peptide coupling reagents. Org. Prep. Proced. Int.:  2001; 33(3): 202:203-303
7. Kaiser E., Colescott R. L., Bossinger C. D., Cook P. I.: Color test for detection on free terminal amino groups in the solid-phase synthesis of peptides. Anal. Biochem. 1970 Apr;34(2):595-598 [PubMed]

Please cite this article as: Paypanova T, Hristova T, Cherkezova R. SYNTHESIS OF OLIGOPEPTIDES OF DEFINED FRAGMENT COMPOSITION. J of IMAB 2011; 17(1):127-129. doi: 10.5272/jimab.2011171.127

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