Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2014, vol. 20, issue 1
Subject Collection: Oral and Dental Medicine
Pages: 464-468
DOI: 10.5272/jimab.2014201.464
Published online: 10 March 2014

J of IMAB 2014 Jan-Jun;20(1):464-468
Vladimir E. Panov,1 Assya Krasteva2 Corresponding Autor, Adriana Z. Krasteva3, Aneta Ivanova4, Anton Panov5, Zahariy Krastev4.
1) Department of Conservative dentistry and oral pathology, Faculty of Dental Medicine, Medical University Varna, Bulgaria
2) Department of Diagnostic Imaging and Oral diagnostic, Faculty of Dental medicine, Medical University, Sofia, Bulgaria
3) Department of Pharmacology and Toxicology, Faculty of Pharmacy, Medical University - Sofia, Bulgaria
4) Clinic of Gastroenterology, University Hospital "St. Ivan Rilski", Sofia, Bulgaria
5) Dentist. Private practice, Sofia, Bulgaria.

Objective: Calprotectin is a calcium binding heterocomplex protein which appear to have regulatory functions in the inflammatory process. Epithelial cells which expressing calprotectin are more resistant to bacterial invasion . In acute phase inflammatory reactions calprotectin is detectable in elevated levels.
The aim of the present study is to detect the calprotectin level in saliva in patients with periodontal disease (chronic and aggressive periodontitis and gingivitis) and to follow calprotectin level during azithromycin treatment.
Methods and materials: In nine healthy patients without systemic disease and malignancy whole unstimulated saliva was investigated.  Salivary calprotectin levels were measured by enzyme-linked-immunosorbent using a commercial kit (ELISA Hycult Biotech). Azithromycin treatment was taken as 500 mg (2 x 250 mg tabls.) once daily at 10.00 am for 4 consecutive days. 
Results: At baseline Day levels of calprotectin ranged between 1.45 and 2.97; median 2.19. On Day 5 (first day after azithromycin treatment) the salivary calprotectin levels decreased in 6 of the patients. The measured values at Day 10 were more similar to those at Day 5, than those at Day zero. This was confirmed by the values of the average median of calprotectin.
Conclusion: We present the first study with the application of antibiotic and measurement the calprotectin levels before, during and after azithromycin intake with no side effects reported. 
Measuring calprotectin levels could benefit the monitoring of antibiotic treatment efficacy in patients with gingivitis and periodontitis.

Key words: calprotectin; saliva; periodontal disease;

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Please cite this article as:
Panov VE, Krasteva A, Krasteva AZ, Ivanova A, Panov A, Krastev Z. Azithromycin decrease saliva calprotectin in patients with periodontal diseases. J of IMAB. 2014 Jan-Jun;20(1):464-468. doi: 10.5272/jimab.2014201.464.

Correspondence to: Dr.  Assya Krasteva, Department of Diagnostic Imaging and Oral diagnostic, Faculty of Dental Medicine, Medical University – Sofia; 1, G. Sofiyski blvd., Sofia, Bulgaria; E-mail:

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Received: 20 December 2013
Published online: 10 March 2014

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