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Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2016, vol. 22, issue 2
Subject Area: Dental Medicine
Pages: 1127-1131
DOI: 10.5272/jimab.2016222.1127
Published online: 31 May 2016
J of IMAB 2016 Apr-Jun;22(2):1127-1131
SERUM HEPCIDIN REFERENCE RANGE, GENDER DIFFERENCES, MENOPAUSAL DEPENDENCE AND BIOCHEMICAL CORRELATES IN HEALTHY SUBJECTS
Biljana Ilkovska1 
, Bisera Kotevska2, Georgi Trifunov2, Branimir Kanazirev3.
1) Department of Medical Biochemistry, PHO Clinical Hospital Bitola, Macedonia
2) Department of Dermatology and Venereology and Otorhinolaryngology, Tokuda Hospital, Sofia, Bulgaria
3) Department of Medicine, Medical University, Varna, Bulgaria.
ABSTRACT:
Background: Hepcidin has emerged as the central regulatory molecule of iron homeostasis. Iron deficiency and iron overload play a major role in molecular insights of many disease states and serum hepcidin normal values and biochemical correlations are of substantial importance.
Objective: The aim of this study is to examine the serum hepcidin reference range, gender and age differences, menopausal dependence and biochemical correlates in healthy subjects.
Methods: Serum hepcidin concentration was measured with a competitive enzyme-linked immunosorbent assay (DRG Hepcidin-25 ELISA Kit) together with hemoglobin, hematocrit, serum iron, transferrin and C-reactive protein in 120 healthy subjects both men and pre- and post-menopausal women.
Results: Normal serum hepcidin values were found in the range of 1,23 – 36,46 ng/mL (mean 9,25 ± 6,45 ng/mL).There were statistically significant differences in measured hepcidin levels between men (12,34 ± 7,37 ng/mL) and women (6,16 ± 3,2 ng/mL) (p<0.01) and between pre- menopausal (5,51 ± 2,8 ng/mL) and post-menopausal women (7,29 ± 3,59 ng/mL) ( p<0,05). Strong correlations were found with serum ferritin and hemoglobin but not with serum iron, transferrin and CRP. No 5-year age interval differences were deemed significant.
Conclusion: Serum hepcidin concentration varied substantially between subjects, which is reflected in wide reference ranges. Serum hepcidin levels were gender and menopausal status related and were in correlation with hemoglobin and serum ferritin in healthy subjects.
Key words: hepcidin, range, gender, menopause, healthy subjects,
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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Ilkovska B, Kotevska B, Trifunov G, Kanazirev B. Serum hepcidin reference range, gender differences, menopausal dependence and biochemical correlates in healthy subjects. J of IMAB. 2016 Apr-Jun;22(2):1127-1131. DOI: http://dx.doi.org/10.5272/jimab.2016222.1127.
Correspondence to: Biljana Ilkovska, Department of Medical Biochemistry PHO Clinical Hospital dr. Trifun Panovski; st. Partizanska b.b, 7000 Bitola, R. Macedonia; E-mail: drbiljanailkovska@yahoo.com
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Received: 18 March 2016
Published online: 31 May 2016