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Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2017, vol. 23, issue 2
Subject Area: Medicine
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DOI: 10.5272/jimab.2017232.1603
Published online: 26 June 2017
Original article
J of IMAB 2017 Apr-Jun;23(2):1603-1606
AN OPTIMIZED GC-MS METHOD FOR AMPHETAMINES IDENTIFICATION
Georgi Bonchev1
, Snezha Zlateva1,2, Petko Marinov1,2, Ivaylo Vazharov3
1) Clinic for Intensive Treatment of Acute Intoxications and Toxicoallergies, Naval Hospital – Varna, Military Medical Academy, Bulgaria
2) Department of Pharmacology, Toxicology and Pharmacotherapy, Faculty of Pharmacy, Medical University – Varna, Bulgaria
3) Clinic of Internal Diseases, Naval Hospital – Varna, Military Medical Academy, Bulgaria.
ABSTRACT:
Purpose: To develop a reliable method for qualitative determination of amphetamines in biological samples, that combines simplicity, sensitiveness and robustness. Optimization of the method should be carried out in order to meet the needs of forensic expertise as daily routine.
Material/Methods: Analytical identification was done by menas of gas chromatography – mass spectrometry. Validation was carried out using certified analytical standards.
Results: Sample preparation procedure has been simplified. Only common reagents are needed. Solid phase extraction (SPE) has been used for initial purification of biological samples. Additional derivatization (PFPA) has been shown to be of major importance. Limit of detection (LOD) in blood samples has been determined to be as low as 25 ng/mL for amphetamine (AMP) and 10 ng/mL for methamphetamine (MET). Results are given within 100 minutes.
Keywords: samphetamine, methamphetamine, solid phase extraction, analytical toxicology, drugs of abuse, forensic expertise,
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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Bonchev G, Zlateva S, Marinov P, Vazharov I. An optimized GC-MS method for amphetamines identification. J of IMAB. 2017 Jan-Mar;23(2):1603-1606. doi: 10.5272/jimab.2017232.1603
Correspondence to: Georgi Bonchev, PhD, Head, Laboratory of Analytical Toxicology, Military Medical Academy, Naval Hospital – Varna; 3, Hristo Smirnenski Blvd., 9010 Varna, Bulgaria; E-mail: toxilab.varna@abv.bg
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Received: 03 April 2017
Published online: 26 June 2017