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Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2017, vol. 23, issue 4
Subject Area: Medicine
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DOI: 10.5272/jimab.2017234.1765
Published online: 28 November 2017
Original article
J of IMAB 2017 Oct-Dec;23(4):1765-1768
A UHPLC METHOD FOR SERTRALINE DETERMINATION
Georgi Bonchev1 
, Snezha Zlateva1,2, Petko Marinov1,2, Marieta Yovcheva1, Ivaylo Vazharov3,
1) Clinic for Intensive Treatment of Acute Intoxications and Toxicoallergies, Naval Hospital – Varna, Military Medical Academy, Bulgaria
2) Department of Pharmacology, Toxicology and Pharmacotherapy, Faculty of Pharmacy, Medical University – Varna, Bulgaria
3) Clinic of Internal Diseases, Naval Hospital – Varna, Military Medical Academy, Bulgaria.
ABSTRACT:
Purpose: To develop and implement a UHPLC method for quantitative determination of sertraline in biological samples – mostly human blood and urine.
Material&Methods: Blood and urine samples available from Laboratory of analytical toxicology and Clinic for intensive treatment of acute intoxications and toxicoallergies were used during method validation and case monitoring. Analytical identification of sertraline and/or metabolites was done by GC-MS. Gas chromatography coupled with flame ionization detection was used for alcohols/volatiles screening of clinical samples. Ultra high-performance liquid chromatography system in tandem with diode-array detector has been used as the main quantitative instrument.
Results: After critical consideration of available reference data a carefully set of experimental conditions for sertraline extraction and UHPLC determination were adopted and optimized. Preliminary liquid-phase sample purification was applied. Zorbax Extend-C18 column (150 x 4.6 mm, 5 µm) was used under isocratic conditions with phosphate buffer (pH 2.7; 10 mM) containing 1.5 ml L–1 triethylamine – acetonitrile (65:35, v/v) at 20oC, at the flow-rate of 1.0 mL/min and UV detection at 220 nm. This method was validated for the determination of sertraline in human plasma/serum samples (70% recovery).
Conclusions: A simple yet sensitive and reliable method for sertraline determination was introduced. Linearity over 20-1000 ng mL–1 range was shown; LOQ was 20 ng mL–1. The method was clinically applied for monitoring the blood sertraline levels during a course of detoxication of a female patient.
Keywords: sertraline, Zoloft, HPLC determination, drug monitoring,
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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Bonchev G, Zlateva S, Marinov P, Yovcheva M, Vazharov I. A UHPLC method for sertraline determination. J of IMAB. 2017 Oct-Dec;23(4):1765-1768. DOI: 10.5272/jimab.2017234.1765
Correspondence to: Georgi Bonchev, PhD, Head, Laboratory of Analytical Toxicology, Military Medical Academy, Naval Hospital – Varna; 3, Hristo Smirnenski Blvd., 9010 Varna, Bulgaria; E-mail: toxilab.varna@abv.bg
REFERENCES:
1. Baldessarini RJ. Drug therapy of depression and anxiety disorders. In: Brunton LL, (editor). The pharmacological basis of therapeutics. 11th ed. New York: The McGraw-Hill Companies, 2006; p. 445.
2. Thakore JH, Berti C, Dinan TG. An open trial of adjunctive sertraline in the treatment of chronic schizophrenia. Acta Psychiatr Scand. 1996 Sep;94(3):194-7. [PubMed] [CrossRef]
3. Grohol JM. Top 25 Psychiatric Medication Prescriptions for 2013. Psych Central. 2016. [Internet]
4. Potter WZ, Hollister LE. Antidepressant agents. In: Katzung BG (Ed.), Basic and clinical pharmacology. 10th ed. New York: The McGraw-Hill Companies; 2007. 475 p.
5. Sanchez C, Reines EH, Montgomery SA. A comparative review of escitalopram, paroxetine, and sertraline: Are they all alike? Int Clin Psychopharmacol. 2014 Jul;29(4):185-96. [PubMed] [CrossRef]
6. Taylor D, Paton C, Shitij K. The Maudsley prescribing guidelines in psychiatry. 12th ed. West Sussex: Wiley-Blackwell; 2015. 760 p.
7. White N, Litovitz T, Clancy C. Suicidal antidepressant overdoses: a comparative analysis by antidepressant type. J Med Toxicol. 2008 Dec;4(4):238-50. [PubMed]
8. DeVane CL, Liston HL, Markowitz JS. Clinical pharmacokinetics of sertraline. Clin Pharmacokinet. 2002; 41(15):1247-66. [PubMed]
9. Schulz M, Iwersen-Bergmann S, Andresen H, Schmoldt A. Therapeutic and toxic blood concentrations of nearly 1,000 drugs and other xenobiotics. Critical Care. 2012 Jul 26;16(4):R136. [PubMed] [CrossRef]
10. Singhvi I, Chaturvedi SC. Visible spectrophotometric and HPLC methods for the estimation of sertraline hydrochloride from tablet formulations. Indian J Pharm Sci. 2000 Nov-Dec;62(6):468-70. [Internet]
11. Rahman MA, Iqbal Z, Mirza MA, Hussain A. Estimation of sertraline by chromatographic (HPLC-UV 273 nm) technique under hydrolytic stress conditions. Pharm Methods. 2012 Jul;3(2):62-7. [PubMed] [CrossRef]
12. Mandrioli R, Saracino MA, Ferrari S, Berardi D, Kenndler E, Raggi MA. HPLC analysis of the second-generation antidepressant sertraline and its main metabolite N-desmethylsertraline in human plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 19;836(1-2):116-9. [PubMed] [CrossRef]
13. Patel J, Spencer EP, Flanagan RJ. HPLC of sertraline and norsertraline in plasma or serum. Biomed. Chromatogr. 1996 Nov-Dec;10(6):351-4. [PubMed] [CrossRef]
14. Ferrarini A, Huidobro AL, Pellati F, Barbas C. Development and validation of a HPLC method for the determination of sertraline and three non-chiral related impurities. J Pharm Biomed Anal. 2010 Oct 10;53(2):122-9. [PubMed] [CrossRef]
15. Lucca A, Gentilini G, Lopez-Silva S, Soldarini A. Simultaneous determination of human plasma levels of four selective serotonin reuptake inhibitors by high-performance liquid chromatography. Ther Drug Monit. 2000 Jun;22(3):271-6. [PubMed]
16. Farshchia A, Ghiasia G, Bahramia G. High performance liquid chromatography determination of sertraline in human. IJPS. 2009 Summer;5(3):171-8. [Internet]
17. Ravindra N. Spectrophotometric methods for quantitative estimation of sertraline hydrochloride from tablet formulation. IJPAR. 2014 Oct-Dec;3(4):422-5. [Internet]
18. Kim KM, Jung BH, Choi MH, Woo JS, Paeng KJ, Chung BC. Rapid and sensitive determination of sertraline in human plasma using gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Apr 5;769(2):333-9. [PubMed].
Received: 12 June 2017
Published online: 28 November 2017