head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2018, vol. 24, issue3
Subject Area: Medicine
DOI: 10.5272/jimab.2018243.2129
Published online: 28 August 2018

Original article

J of IMAB. 2018 Jul-Sep;24(3):2129-2132
Tsvetelina Kostadinova1ORCID logo Corresponding Autoremail, Liliya Ivanova2ORCID logo, Zhivka Stoykova2ORCID logo, Tatina Todorova3ORCID logo, Liana Gercheva4ORCID logo, Dobromir Staykov5ORCID logo, Denitsa Tsaneva6ORCID logo.
1) Education and Research Sectors of Medical Laboratory Assistant, Medical College, Medical University, Varna, Bulgaria
2) Laboratory of Clinical Virology – University hospital St. Marina, Medical University, Varna, Bulgaria
3) Department of Preclinical and Clinical Sciences, Faculty of Pharmacy, Medical University Varna, Bulgaria
4) Clinic of Hematology at the University Hospital St. Marina, Medical University Varna, Bulgaria
5) Regional Center of Transfusion Hematology - Varna, Bulgaria
6) Department of Microbiology and Virology, Faculty of Medicine, Medical University Varna, Bulgaria.

Epstein-Barr virus (EBV) reactivates during immunosuppression (IS) and immune deficiency. The introduction of stem cell transplantation and the development of transplantology require compliance with criteria for assessing the risk of reactivation of latent viral infections, including EBV. There are no published EBVDNA findings in Bulgaria for such patient groups,
Aim: The aim in this study is to assess the possible benefit of EBV PCR testing in immunosuppressed individuals.
Materials and Methods: We investigated 50 immunosuppressed patients – 28 with various haematological diseases, 17 after kidney transplantationand5 patients with autologous stem cell transplantation (HSCT). Patients were first tested in an indirect ELISA to detect anti-VCA IgM/IgG (Euroimmun, Luebeck, Germany) and then in quantitative PCR (Sacace Biotechnologies S.r.l., Como, Italy).
Results: We found ЕВV DNAin 14.0% (95% CI:5.8% - 26.7%, n=7) of all tested patients. The Real time PCR results were in the range 100-500 copies/ml at the lower limit of the 500 copies/ml test positivity. The highestistheproportionofpatientswithhaematological diseases (21.4%), predominantly with AML.
Conclusion: We found a relatively small proportion of IS patients with detectable EBV DNA. For HSC-transplanted patients, we anticipate probable reactivation or reinfection in one patient, who was anti-VCA IgG positive in the primary study.

Keywords: Epstein-Barr virus, Immunosuppressed patients, Acute myeloid leukaemia, Kidney transplantation, Autologous hematopoietic stem cells transplantation, Real time PCR,

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Please cite this article as: Kostadinova T, Ivanova L, Stoykova Z, Todorova T, Gercheva L, Staykov D, Tsaneva D. Investigation of immunosuppressed patients for the presence of EBV DNA in Real time PCR. J of IMAB. 2018 Jul-Sep;24(3):2129-2132. DOI: 10.5272/jimab.2018243.2129

Corresponding AutorCorrespondence to: Tsvetelina Kostadinova, MD, PhD, Education and Research Sectors of Medical Laboratory Assistant, Medical College, Medical University; 84 Tsar Osvoboditel Blvd., Varna, Bulgaria; E-mail: ckostadinova@abv.bg,

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Received: 22 May 2018
Published online: 28 August 2018

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