head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2018, vol. 24, issue4
Subject Area: Medicine
DOI: 10.5272/jimab.2018244.2250
Published online: 3 November 2018

Original article

J of IMAB. 2018 Oct-Dec;24(4):2250-2253
Poli Radusheva1ORCID logo, Krastena Nikolova1ORCID logo Corresponding Autoremail, Nadezhda Petkova2ORCID logo, Radoslava Gabrova3, Darina Naydenova4ORCID logo,
1) Department of Physics and Biophysics, Faculty of Pharmacy, Medical University - Varna, Bulgaria
2) Department of Organic Chemistry and Inorganic Chemistry, Technological Faculty, University of Food Technologies- Plovdiv, Bulgaria
3) Department of Computer systems and Technologies, Technical Faculty, University of Food Technologies- Plovdiv, Bulgaria
4) Department of Preclinical and Clinical sciences, Faculty of Pharmacy, Medical University - Varna, Bulgaria.

Introduction: The natural woody syrup is a typical product from the Canary Islands and America. It is often used for health food supplement for children, athletes and elderly because it has well-pronounced antioxidant properties. The interest in this syrup is increasing because it is often used in homeopathic medicine and consists of phenolic content. Antioxidants reduce cell damaging effects of free radicals. They are more effective in vitro antioxidants than vitamins D and C and have a significant effect towards protection of unwanted in vivo oxidation of proteins and lipids.
Objective: The natural woody syrup is prepared from Canadian maple syrup and syrup from 5 types of palms from Southeast Asia. The nutritional properties (such as the content of sugars) were analyzed. The antioxidant activity, total phenolic content and optical properties have been investigated.
Purpose: The aim of this study is to explore the relations between fluorescence in natural woody syrup and its total phenolic content and total antioxidant activity in view of the usefulness of this product for human health and to determine their nutrient composition by the physico-chemical properties.
Materials and methods: The natural woody syrup will be investigated by using methods of applied photonic, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing antioxidant power (FRAP) methods for antioxidant activity, Folin–Ciocalteu reagent for total phenols and High-Performance Liquid Chromatography Refractive Index Detector HPLC-RID analysis for determination of the sugar content.
Results: The dependence between antioxidant activity and total phenolic content was established. The sample has a small quantity of flavonoids, and for this reason, there is not the distinct peak at 380 nm. Excitation in the visible region is suitable for distinguishing the riboflavin and pigments.
Conclusions: The fluorescence of the syrup was correlated with their antioxidant activity. Two emission fluorescence maxima have been observed:
-The first one is attributed to the riboflavin in the region 550 nm - 580 nm
-The second one is connected with the pigments similar to the chlorophyll at about 690 nm.

Keywords: natural woody syrup, fluorescence spectroscopy, phenolic content, antioxidant activity, sugar content,

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Please cite this article as: Radusheva P, Nikolova K, Petkova N, Gabrova R, Naydenova D. Optical properties and chemical characterization of woody syrup by using methods of applied photonics. J of IMAB. 2018 Oct-Dec;24(4):2250-2253. DOI: 10.5272/jimab.2018244.2250

Corresponding AutorCorrespondence to: Krastena Todorova Nikolova, Department of Physics and Biophysics, Faculty of Pharmacy, Medical University – Varna; 84, Tsar Osvoboditel Blvd., Varna, Bulgaria; E-mail: kr.nikolova@abv.bg

1. Ball DW. The Chemical Composition of Maple Syrup. J Chem Educ. 2007;84(10):1647. [Crossref]
2. Tanako H. Investigation of chemical and Physical properties of Southwestern Wisconsin maple syrup. Journal of student research. 2006;97-107.
3. Stintzing FC, Nerbach KM, Mosshammer MR, Carle R, Yi W, Sellappan S, et al. Color, betalain pattern, and antioxidant properties of cactus pear (Opuntia spp.) clones. J. Agric Food Chem. 2005 Jan 26;53(2):442-451. [PubMed] [CrossRef]
4. Ivanov IG, Vrancheva RZ, Marchev AS, Petkova NT, Aneva YI, Denev PP, et al. Antioxidant activities and phenolic compounds in Bulgarian Fumaria species. Int J Curr Microbiol App Sci. 2014 Feb;3(2):296-306.
5. Benzie IF, Srain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem. 1996 Jul 15;239(1):70-76. [PubMed] [CrossRef]
6. Petkova N, Petrova I, Ivanov I, Mihov R, Hadjikinova R, Ognyanov M, et al. Nutritional and antioxidant potential of carob (Ceratonia siliqua) flour and evaluation of functional properties of its polysaccharide fraction. J Pharm Sci & Res. 2017; 9(11):2189-2195.
7. Kermasha S, Goetghebeur M, Dumont J. Determination of phenolic compound profiles in maple products by high –perfomance liquid chromatography. J Agric Food Chem. 1995; 43(3):708-716. [CrossRef]
8. Sikorska E, Gorecki T, Khmelinskii IV, Sikorski M, De Keukeleire D. Fluorescence spectroscopy for characterization and differentiation of beers. J Inst Brewing. 2004; 110(4):267-275. [CrossRef]
9. Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends in Plant Science. 1997 Apr 1;2(4):152-159. [CrossRef]
10. Mitev D, Vlakhov E, Vlakhov T, Peshev D, Hristova-Avakumova N, Hadjimitova V. Effect of Nanofiltration on the Antioxidant Properties of Crataegus monogyna Curative Extracts. Bulg J Phys. 2016; 43(4):311-319.
11. Hadjimitova V, Hristova-Avakumova N, Traykov T. Relationship between antioxidant properties and GC-MS component composition of extracts from flowers, leaves and fruits of Crataegus Oxycanta. J Advances in Agriculture. 2015;4(1):310-315.
12. Luis G, Rubio C, Gutierrez AJ, Hernandez C, Gonzalez-Weller D, Revert C, et al. Palm tree syrup: nutritional composition of a natural edulcorant. Nutr Hosp. 2012 Mar-Apr;27(2):548-52. [PubMed] [CrossRef].

Received: 22 May 2018
Published online: 23 November 2018

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