head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2019, vol. 25, issue4
Subject Area: Dental Medicine
DOI: 10.5272/jimab.2019254.2822
Published online: 09 December 2019

Original article

J of IMAB. 2019 Oct-Dec;25(4):2822-2827;
Nadezhda Mitova1ORCID logo Corresponding Autoremail, Maya Rashkova1ORCID logo, Christina Popova2ORCID logo,
1) Department of Pediatric Dentistry, Faculty Dental Medicine, Medical University – Sofia, Bulgaria.
2) Department of Periodontology, Faculty Dental Medicine, Medical University – Sofia, Bulgaria.

Introduction: Gingival inflammations relating to an increase in the quantity of dental plaque are most frequently seen in children and young adults. Their spread and severity increase with age and reach their peak during puberty.
Aim - To study the main microorganisms of the subgingival microflora in children in puberty (10 – 14 years of age).
Material and methods: 60 children aged 10-14 years subjected to monitoring- 30 without gingivitis (up to 25% PBI) and good oral hygiene and 30 children with plaque-induced gingivitis (over 50% PBI). A PCR – Real-Time method was used for identifying the main subgingival microorganisms and determining their quantities. Samples were taken with paper pins from the gingival sulcus of six teeth – three molars, two canines and one incisor (16, 13, 11, 26, 36, 43). Nine control strains were studied (a comprehensive sample). Samples were sent for research in standardized containers.
Results: The results of this study show that the total quantity of subgingival microorganisms increases in the case of worsening oral hygiene and an increase in the quantity of dental biofilm. Six of the nine subgingival microorganisms tested were encountered in all children between the ages of 10 and 14. The remaining three species of microorganisms were isolated only from the children with gingivitis.
Conclusion: During gingival inflammationshe subgingival microflora becomes more complex and from it can be isolated microorganisms from the red complex (P.gingivalis, T.denticola, T.forsythia).

Keywords: subgingival microorganisms, PCR-Real Time, periodontal diseases,

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Please cite this article as: Mitova N, Rashkova M, Popova Ch. Studying subgingival microorganisms in children with gingivitis in puberty. J of IMAB. 2019 Oct-Dec;25(4):2822-2827. DOI: 10.5272/jimab.2019254.2822

Corresponding AutorCorrespondence to: Nadezhda Georgieva Mitova, Department of Pediatric Dentistry, Faculty of dental medicine, Medical University – Sofia; 1, Georgi Sofiisky str., Sofia, Bulgaria; E-mail: nadia_bm@abv.bg

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Received: 28 May 2019
Published online: 09 December 2019

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