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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2020, vol. 26, issue1
Subject Area: Medicine
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DOI: 10.5272/jimab.2020261.2905
Published online: 12 February 2020
Original article
J of IMAB. 2020 Jan-Mar;26(1):2905-2909
CLINICAL STUDY OF GENE EXPRESSION PROFILE AND SOME OF THE MOST COMMON CHROMOSOMAL ABERRATIONS IN UNTREATED B-CHRONIC LYMPHOCYTIC LEUKAEMIA PATIENTS
Vanya Slavcheva1
, Georgi Balatzenko2, Svetlana Angelova2, Emilia Naseva3, Tzvetan Lukanov4, Nikolai Tzvetkov1,
1) Clinic of Haematology, University Hospital, Medical University, Pleven, Bulgaria.
2) National Hematological Hospital, Lab of cytogenetics and molecular biology, Sofia, Bulgaria.
3) Department of Health Economics, Faculty of Public Health, Medical University, Sofia, Bulgaria.
4) Immunological laboratory, University Hospital, Medical University, Pleven, Bulgaria.
ABSTRACT:
Aim: Chronic lymphocytic leukaemia (CLL) is one of the most common leukaemias in adults. Unlike myeloid and acute lymphoblastic leukaemia, treatment can be delayed after diagnosis. Exploration of the unique biology of the disease has led to the identification of more than 35 prognostic factors reported in the literature. In practice, however, the number of validated clinical and biological prognostic factors is significantly smaller. The purposes of our study are: to investigate the distribution of untreated CLL- patients according to Binet staging as well as to analyse the frequency of one of the most common chromosomal aberrations in the group. To study the correlation between mutational status (determined by evaluation of lipoprotein lipase/ ADAM29 ratio), and the identified chromosomal aberrations in the group of 48 untreated patients.
aterials and methods: We studied 48 patients with a confirmed flowcytometric diagnosis B-CLL, using the 2008 IWCLL-NCI criteria. The interphase fluorescence in situ hybridization (FISH) was applied to determine the chromosomal abnormalities. Analysis of mutational status was performed as described by Pablo Oppezzo with slight modification. Documentary and statistical methods were used for data processing by Fisher`s Exact test.
Conclusion: We found that a large proportion of patients were diagnosed with early-stage A of the disease. In most untreated CLL- patients isolated del13q was detected. Statistically, a significant association was found between del13q, del11q, del17p / p53 and mutational status.
Keywords: lymphocytic leukaemia, lipoprotein lipase, ADAM29, del13q14, prognosis,
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Please cite this article as: Slavcheva V, Balatzenko G, Angelova S, Naseva E, Lukanov Tz, Tzvetkov N. Clinical study of gene expression profile and some of the most common chromosomal aberrations in untreated B-chronic lymphocytic leukaemia patients. J of IMAB. 2020 Jan-Mar;26(1):2905-2909.
DOI: 10.5272/jimab.2020261.2905
Correspondence to: Vanya Slavcheva, MD. Clinic of Hematology, University hospital, Medical University Pleven; 8 A, G. Kochev blvd., Pleven 5800, Bulgaria; E-mail: slavcheva05@gmail.com
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Received: 10 June 2019
Published online: 12 February 2020