head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2020, vol. 26, issue3
Subject Area: Dental Medicine
DOI: 10.5272/jimab.2020263.3234
Published online: 13 July 2020

Original article

J of IMAB. 2020 Jul-Sep;26(3):3234-3240
Antoaneta Mlachkova1ORCID logo, Velitchka Dosseva-Panova1ORCID logo Corresponding Autoremail, Christina Popova1ORCID logo, Maja Kicheva2
1) Department of Periodontology, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria.
2) PhD, biochemist, Progene Ltd., Sofia, Bulgaria.

Background: Periodontitis is a chronic inflammatory disruption of the supportive periodontal tissue. It is known that the chronic periodontitis is initiated by the increased level of specific bacteria, but the progression of the disease is defined by characteristics of the host response to the bacterial load. There is evidence that individual genetic, systemic and environmental risk factors can influence the course of the disease. Some genes and intergenic relations are in connection with the modification of the periodontal disease, and so they change the clinical course, the severity and the prognosis of the decease.
Purpose: The aim of the current investigation is to establish the presence of IL-8 gene polymorphism and serum levels to IL- 8 in patients with chronic periodontitis.
Material and methods: In the recent study, 35 patients (24 females and 11 males) are included with diagnosis moderate to severe periodontitis. Periodontal important parameters such as PD, CAL and Bone loss are included. The level of IL- 8 in a patient’s serum is tested by ELISA (enzyme-linked immunosorbent assay). The presence of IL- 8 polymorphism in loci rs4073 (-251A/T) and rs2227307 (-396T/G) is established by polymerase chain reaction (PCR).
Results: The analysis of the current assay recognized a statistically significant correlation between: HI and PBI- severity and HI and BOP; a significant correlation between PBI- severity and PD average and Bone loss; the strong correlation between BOP and Bone loss; the significant correlation between PD 3- 5mm and PD ≥ 7mm and  CAL average, also a strong correlation between PD ≥ 7mm and Bone loss. The results have shown that the biggest interrelationship observed between the presence of GT/AT genotype and TT/TT genotype and distribution of PD 5-7 mm and PD ≥ 7 mm (p<0.09).
Conclusions: In the recent study have shown that by reason of the heterogeneous results achieved in the group investigated by us we can definitely support the hypothesis that the presence of allele A for rs407 (-251A/T) and allele G for rs2227307 (-396T/G) is strongly related with expression of severe periodontitis.

Keywords: gene polymorphism, interleukin-8, susceptibility, periodontitis, enzyme-linked immunosorbent assay, polymerase chain reaction,

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Please cite this article as: Mlachkova A, Dosseva-Panova V, Popova C, Kicheva M. The presence of Il-8 gene polymorphism at (-251A/T) and (-396T/G) position is related with susceptibility to periodontitis development. J of IMAB. 2020 Jul-Sep;26(3):3234-3240. DOI: 10.5272/jimab.2020263.3234

Corresponding AutorCorrespondence to: Velitchka Dosseva-Panova, Department of Periodontology, Faculty of Dental Medicine, Medical University – Sofia; 1, George Sofiiski str., 1431 Sofia, Bulgaria; E-mail: vdosseva@yahoo.com

1. Mlachkova А, Popova Chr, Kicheva M. [Severity of chronicperiodontitis inrelatedwithIL-1B gene.] Problems of Dental Medicine. 2001. 37 (1): 6-14.[in Bulgarian]
2. Popova Chr, DossevaV, Mlachkova A. Correlation of gene expression of main inflammatory cytokines and the severity of chronic periodontitis. J of IMAB.2014 Jul-Sep;20(3):563-566. [Crossref]
3. Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al. Host Response Mechanisms in Periodontal Diseases. J Appl Oral Sci. 2015 May-Jun;23(3):329-55. [PubMed] [Crossref]
4. Ebersole JL, Nagarajan R, Akers D, Miller CS. Targeted salivary biomarkers for discrimination of periodontal health and  disease(s).  Front  Cell  Infect  Microbiol.  2015 Aug 19;5:62. [PubMed] [Crossref]
5. Laine ML, Loos BG, Crielaard W. Gene polymorphisms in chronic periodontitis. Int J Dent. 2010; 2010: 324719. [PubMed] [Crossref]
6. Kornman KS, di Giovanni FS. Genetic variations in cytokine expression: a risk factor for severity of adult periodontitis. Ann Periodontol. 1998 Jul;3(1):327-38. [PubMed] [Crossref]
7. Karimbux NY, Saraiya VM, Elangovan S, Allareddy V, Kinnunen T, Kornman KS, et al. Interleukin-1 Gene Polymorphisms and Chronic Periodontitis in Adult Whites: A Systematic Review and Meta-Analysis. J Periodontol. 2012 Nov;83(11):1407-19. [PubMed] [Crossref]
8. Linhartova P, Cernochova P, Izakovicova Holla L. IL1 Gene Polymorphisms in Relation to External Apical Root Resorption Concurrent With Orthodontia. Oral Dis. 2013 Apr;19(3):262-70. [PubMed] [Crossref]
9. Loos BG, John RP, Laine ML. Identification of Genetic Risk Factors for Periodontitis and Possible Mechanisms of Action. J Clin Periodontol. 2005; 32(Suppl. 6):159-79. [PubMed] [Crossref]
10. Nikolopoulos GK, Dimou NL, Hamodrakas SJ, Bagos PG. Cytokine Gene Polymorphisms in Periodontal Disease: A Meta-Analysis of 53 Studies Including 4178 Cases and 4590 Controls. J Clin Periodontol. 2008 Sep;35(9):754-67. [PubMed] [Crossref]
11. Talvan ET, Mohor C, Chisnoiu D, Cristea V, Cвmpian RS.  Expression of Interleukin (IL)-1β, IL-8, IL-10 and IL-13 in Chronic Adult Periodontitis Progression. Arch Med. 2017; 9(3):4. [Crossref]
12. Toker H, Gorgun EP, Korkmaz EM. Analysis of IL-6, IL-10 and NF-кB Gene Polymorphisms in Aggressive and Chronic Periodontitis. Cent Eur J Public Health. 2017; 25(2):157-162. [Crossref]
13. Dosseva-Panova V, Mlachkova A, Popova Ch, Kicheva M. Evaluation of interleukin-6, Lymphotoxin-a and TNF-a gene polymorphisms in chronic periodontitis. J of IMAB. 2015 Jul-Sep;21(3):868-875. [Crossref]
14. Duff W. Molecular genetics of cytokines: Cytokines in chronic inflammatorydisease. In: Thompson A, Ed. The cytokine handbook. 1994; 2nd edition: 21-30. London: Academic Press.
15. Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol. 2011 Mar;38(Suppl 11):60-84. [PubMed] [Crossref]
16. Majumder P, Panda SK, Ghosh S, Kumar Dey S. Interleukin Gene Polymorphisms in Chronic Periodontitis: A Case-Control Study in the Indian Population. Arch Oral Biol. 2019 May;101:156-164. [PubMed] [Crossref]
17.McGee JM, Tucci MA, Edmundson TP, Serio CL, Johnson RB. The Relationship Between Concentrations of Proinflammatory Cytokines Within Gingiva and the Adjacent Sulcular Depth.1998 Aug;69(8):865-71. [PubMed] [Crossref]
18. Michalowicz BS, Diehl SR, Gunsolley JC, Sparks BS, Brooks CN, Koertge TE, et al. Evidence of a Substantial Genetic Basis for Risk of Adult Periodontitis. J Periodontol. 2000 Nov;71(11):1699-707. [PubMed] [Crossref]
19. Teles R, Sakellari D, Teles F, Konstantinidis A, Kent R, Socransky S, et al. Relationships Among Gingival Crevicular Fluid Biomarkers, Clinical Parameters of Periodontal Disease, and the Subgingival Microbiota. J Periodontol. 2010 Jan;81(1):89-98. [PubMed] [Crossref]
20. Guzeldemir-Akcakanat E, Sunnetci-Akkoyunlu D, Orucguney B, Cine N, Kan B, Yilmaz EB, et al. Gene-Expression Profiles in Generalized Aggressive Periodontitis: A Gene Network-Based Microarray Analysis. J Periodontol. 2016 Jan;87(1):58-65. [PubMed] [Crossref]
21. Huang W, He B-Y, Shao J, Jia X-W, Yuan Y-D. Interleukin-1b rs1143627 Polymorphism with Susceptibility to Periodontal Disease. Oncotarget. 2017 May 9;8(19):31406-31414. [PubMed] [Crossref]
22. Suzuki A, Ji G, Numabe Y, Ishii K, Muramatsu M, Kamoi K. Large-scale investigation of genomic markers for severe periodontitis. Odontology. 2004; 92:43–47. [Crossref]
23. Venza I, Visalli M, Cucinotta M, De Grazia G, Teti D, Venza M. Proinflammatory Gene Expression at Chronic Periodontitis and Peri-Implantitis Sites in Patients With or Without Type 2 Diabetes. J Periodontol. 2010 Jan;81(1):99-108. [PubMed] [Crossref]
24. Andia DC, de Oliveira NFP, Letra AM, Nociti FH. Jr, Line SRP, de Souza AP. Interleukin-8 Gene Promoter Polymorphism (rs4073) May Contribute to Chronic Periodontitis. J Periodontol. 2011 Jun;82(6):893-9. [PubMed] [Crossref]
25. Heidari Z, Moudi B, Mahmoudzadeh-Sagheb H. Immunomodulatory Factors Gene Polymorphisms in Chronic Periodontitis: An Overview. BMC Oral Health. 2019 Feb 12;19(1):29. [PubMed] [Crossref]
26. Kim YJ, Viana AC, Curtis KMC, Perez Orrico SR, Cirelli JA, Mendes-Junior CT, et al. Association of Haplotypes in the IL8 Gene With Susceptibility to Chronic Periodontitis in a Brazilian Population. Clin Chim Acta. 2010 Sep 6;411(17-18):1264-8. [PubMed] [Crossref]
27. Kim YJ, Viana AC, Curtis KMC, Orrico SRP, Cirelli JA, Mendes-Junior CT, et al. Association of Haplotypes in the IL8 Gene With Susceptibility to Chronic Periodontitis in a Brazilian Population. Clin Chim Acta. 2010 Sep 6;411(17-18):1264-8.  [PubMed] [Crossref]
28. Chen X, Huang J, Zhong L, Ding C. Quantitative Assessment of the Associations Between interleukin-8 Polymorphisms and Periodontitis Susceptibility. J Periodontol. 2015 Feb;86(2):292-300. [PubMed] [Crossref]
29. Houshmand B, Hajilooi M, Rafiei A, Bidgoli M, Soheilifar S. Evaluation of IL-8 Gene Polymorphisms in Patients With Periodontitis in Hamedan, Iran. Dent Res J (Isfahan). 2012 Jul;9(4):427-32. [PubMed]
30. Sippert EA, de Oliveira e Silva C, Visentainer JEL, Sell AM. Association of Duffy Blood Group Gene Polymorphisms with IL8 Gene in Chronic Periodontitis. PLoS ONE. 2013; 8(12):e83286. [Crossref]
31. Linhartova PB, Vokurka J, Poskerova H, Fassmann A, Holla LI. Haplotype Analysis of interleukin-8 Gene Polymorphisms in Chronic and Aggressive Periodontitis. Mediators Inflamm. 2013; 2013:342351. [PubMed] [Crossref]
32. Khosropanah H, Sarvestani EK, Mahmoodi A, Golshah M. Association of IL-8 (-251 a/T) Gene Polymorphism With Clinical Parameters and Chronic Periodontitis. J Dent (Tehran). 2013 May;10(4):312-8. [PubMed]
33. Yucel-Lindberg T, Brunius G. Epidermal Growth Factor Synergistically Enhances interleukin-8 Production in Human Gingival Fibroblasts Stimulated With interleukin-1beta. Arch Oral Biol. 2006 Oct;51(10):892-8. [PubMed] [Crossref]
34. Ni X-B, Jia C, Yu H-D, Li Y-Q, Zeng X-T, Leng W-D. Comprehensive Analysis of interleukin-8 Gene Polymorphisms and Periodontitis Susceptibility. Oncotarget. 2017 Jul 25;8(30):48996-49004. [PubMed] [Crossref]

Received: 08 July 2019
Published online: 13 July 2020

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