head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2020, vol. 26, issue3
Subject Area: Dental Medicine
DOI: 10.5272/jimab.2020263.3308
Published online: 09 September 2020

Original article

J of IMAB. 2020 Jul-Sep;26(3):3308-3312
Vasil Kalchinov1ORCID logo Corresponding Autoremail, Raina Gergova2ORCID logo
1) Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria.
2) Medical Microbiology, Faculty of Medicine, Medical University, Sofia, Bulgaria.

Purpose: The present study aims to evaluate antimicrobial effect of photosensitizer Fotosan against microorganisms from endodontic flora.
Material and methods: Staphtlococcusaureus ATCC29213 (MSSA), S.aureus ATCC43300 (MRSA), Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 were used as control strains and test bacteria. Several clinical isolates like E. faecalis, Klebsiella pneumoniae, Enterobacter cloaceae, Serratia marcescens, Morganela morganii were used supplementary. Suspensions with concentration 0.5 at MacFarland’s scale and Muller-Hinton agar were used according to criteria of EUCAST 2019. The used Fotosan was with the lowest viscosity. Plates with diameter of  100 millimeters were used in the study. The three filter paper disks were put on inoculated with testing microbial strain agar surface. The disks were consecutively  soaked with 0.5 ml of Fotosan. LED lamp with λ = 630 nm was a source of light. Output power was 200 mW. The power density was 700 mW/cm2. The three disks have been consecutively illuminated, the first for 20 seconds, the second for 40 seconds and the third for 60 seconds. The plates were cultivated for 24 hours at aerobic conditions at 36oC after that.
Results: The zones of inhibition of bacterial growth have varied from 8 to 10 mm for Gram-positive bacteria and 6 to 8 mm for Gram-negative bacteria. 
Conclusion: The presence research has confirmed that photodynamic therapy with Fotosan is an effective method for liquidation of microorganisms, especially with more expression antimicrobial effect against Gram-positive bacteria, which participate more often in endodontic microbial pathology.

Keywords: endodontic microbiology, photodynamic therapy,

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Please cite this article as: Kalchinov V, Gergova R. Investigation of antimicrobial effect of photodynamic therapy with Fotosan in laboratory conditions. J of IMAB. 2020 Jul-Sep;26(3):3308-3312.
DOI: 10.5272/jimab.2020263.3308

Corresponding AutorCorrespondence to: Assist. Prof. Vasil Kalchinov, DMD, PhD; Department of Conservative dentistry, Faculty of Dental Medicine, Medical University of Sofia; 1, Georgi Sofijski Str., 1431 Sofia, Bulgaria; E-mail: vasko_kalchinov@mail.bg

1. Maeda H, Tsukigawa K, Fang J. A Retrospective 30 Years After Discovery of the Enhanced Permeability and Retention Effect of Solid Tumors: Next-Generation Chemotherapeutics and Photodynamic Therapy--Problems, Solutions, and Prospects. Microcirculation. 2016 Apr;23(3):173-82. [PubMed] [Crossref]
2. Plotino G, Grande NM, Mercade M. Photodynamic therapy in endodontics. Int Endod J. 2019 Jun;52(6):760-774.[PubMed] [Crossref]
3. Moreira A, Novaes A, Grisi M, Taba M, Souza S, Palioto D, et al. Antimicrobial Photodynamic Therapy as an Adjunct to Non-Surgical Treatment of Aggressive Periodontitis: A Split-Mouth Randomized Controlled Trial. J Periodontol. 2015 March;86(3):376-386. [PubMed] [Crossref]
4.   Georgieva Tz. [Possibilities of photo-activated disinfection and radiation of ND: YAG LASER for elimination of endodontic microflora.] [dissertation] Medical University of Sofia, (Bulgaria). 2015. 188 p. [in Bulgarian]
5. Ahmad MZ.  Anti-microbial Photo Dynamic Therapy (aPDT) in Endodontics: A Literature Review. Int J Dental Scien Res. 2018 Feb;6(2):25-28. [Crossref]
6. Cieplik F, Deng D, Crielaard W, Buchalla W, Hellwig E, Al-Ahmad A, et al. Antimicrobial Photodynamic Therapy - What We Know and What We Don't. Crit Rev Microbiol. 2018 Sep;44(5):571-589. [PubMed] [Crossref]
7. Hu X, Huang YY, Wang Y, Wang X, Hamblin MR. Antimicrobial Photodynamic Therapy to Control Clinically Relevant Biofilm Infections. Front Microb. 2018 Jun 27;9:1299. [PubMed] [Crossref]
8. Hosseini N, Yazdanpanah S, Saki M, Rezazadeh F, Ghapanchi J, Zomorodian K. Susceptibility of Candida albicans and Candida dubliniensis to Photodynamic Therapy Using Four Dyes as the Photosensitizer.  J Dent (Shiraz). 2016 Dec;17(4):354-360. [PubMed]
9. Ablon G.  Phototherapy with Light Emitting Diodes: Treating a Broad Range of Medical and Aesthetic Conditions in Dermatology. J Clin Aesthet Dermatol. 2018 Feb;11(2):21-27. [PubMed]
10. Sorbellini E, Rucco M, Rinaldi F. Photodynamic and photobiological effects of light-emitting diode (LED) therapy in dermatological disease: an update. Lasers Med Science. 2018 Sep;33(7):1431-1439. [PubMed] [Crossref]
11. Nalawade TM, Bhat KG, Sogi S. Antimicrobial Activity of Endodontic Medicaments and Vehicles using Agar Well Diffusion Method on Facultative and Obligate Anaerobes. Int J Clinic Ped Dent. 2016 Oct-Dec;9(4):335-341.
[PubMed] [Crossref]
12. Omidi S, Hoshyari N, Mirzadeh AR, Hassanabadi ME, Ahajan M, Charati JY, et al.  Comparison of Antibacterial Activity of Three Endodontic Sealers against Enterococcus faecalis. J  Res Med Dent Sci. 2018 Feb;6(1):413-417.
13. Poggio C, Trovati F, Ceci M, Colombo M, Pietrocola G. Antibacterial activity of different root canal sealers against Enterococcus faecalis. J Clin Exp Dent. 2017 Jun 1;9(6):e743-e748. [PubMed] [Crossref]
14. Yadav P, Chaudhary S, Saxena RK, Talwar S, Yadav S. Evaluation of Antimicrobial and Antifungal efficacy of Chitosan as endodontic irrigant against Enterococcus Faecalis and Candida Albicans Biofilm formed on tooth substrate. J Clin Exp Dent. 2017 Mar;9(3):e361-e367. [PubMed] [Crossref]
15.  Rödig T, Endres S, Konietschke F, Zimmermann O, Sydow HG, Wiegand A. Effect of fiber insertion depth on antibacterial efficacy of photodynamic therapy against Enterococcus faecalis in root canals. Clin Oral Investig. 2017 Jun;21(5):1753–1759 [PubMed] [Crossref]
16. Gergova RT, Gueorgieva Tz, Dencheva-Garova M, Krasteva-Panova AZ, Kalchinov V, Mitov I, et al. Antimicrobial activity of different disinfection methods against biofilms in root canals. J Investig Clinic Dent. 2016 Aug;7(3):254-262 [PubMed] [Crossref]
17. Ghasemi M, Etemadia A, Nedaei M, Chiniforush N, Pourhajibagher M. Antimicrobial Efficacy of Photodynamic Therapy Using Two Different Light Sources on the Titanium-Adherent Biofilms of Aggregatibacter Actinomycetemcomitans: An in Vitro Study. Photodiagnosis Photodyn Ther. 2019 Jun;26(3): 85–89. [PubMed] [Crossref]
18. Oruba Z, Labuz P, Macyk W, Chomyszyn-Gajewska M. Periopathogens differ in terms of the susceptibility to toluidine blue O-mediated photodynamic inactivation. Photodiagnosis Photodyn Ther. 2017 Dec;20(12):28-34. [PubMed] [Crossref]
19. Yassaei S, Zandi H, Aghili H, Rafiei E, Mosayebi N. Evaluation of the effect of two types of laser on the growth of Streptococcus mutans. Laser Ther. 2018 Jun 30;27(2):119-123. [PubMed] [Crossref]
20. Afkhami F, Akbari S, Chiniforush N. Entrococcus faecalis Elimination in Root Canals Using Silver Nanoparticles, Photodynamic Therapy, Diode Laser, or Laser-activated Nanoparticles: An In Vitro Study. J Endod. 2017 Feb;43(2):279-282. [PubMed] [Crossref]
21. Kalchinov V, Dimitrov S, Belcheva M. In vitro study of bactericidal effect of antimicrobial agents used in modern endodontics. J of IMAB. 2009; 15(2):79-83 [Crossref]
22. Dimitrov S, Kalchinov V, Belcheva M. Investigation of the bactericidal effect by modified agar - diffusion method. Dent Medicine. 2011; 93 (1):24-31.

Received: 15 August 2019
Published online: 09 September 2020

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