head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2020, vol. 26, issue4
Subject Area: Medicine
DOI: 10.5272/jimab.2020264.3463
Published online: 24 November 2020

Original articles

J of IMAB. 2020 Oct-Dec;26(4):3463-3468
Tsvetan Sokolov1ORCID logo Corresponding Autoremail, Aneliya Manukova2ORCID logo,
1) Department of Orthopedics and Traumatology, MU Pleven, Bulgaria.
2) Department of Electronics, University of Ruse, Ruse, Bulgaria.

OBJECTIVE: To present our problematic skin wounds cases treated with PRP application of that has not responded to the treatment method.
MATERIAL AND METHODS: Out of a total of 154 hospitalized patients with complex skin wounds 83 have been treated with platelet-rich plasma, comprising the Experimental group (EG), and 71 patients with comparable wounds have been treated by utilizing traditional techniques for the respective pathology, comprising the Control Group (CG). Ratings introduced by Cancela AM are used for the evaluation of the respective wound. Statistical analyzes have been performed by using the software SPSS (Statistical Package for the Social Sciences). All cases were treated in a procedure, which includes activated platelet-rich plasma. The treatment algorithm we used is as follows: first, there is surgical treatment and debridement of the wound. Activated plasma is used to infiltrate the wound edges and the fibrin clot is used to fill the wound itself. A sterile dressing soaked with physiological saline is applied. The treatment is repeated on a weekly basis until the wound problem is resolved in full.
RESULTS: The results of our research indicate that 77 (92.78%) of patients enjoy a full recovery, while 6 (7.22%) of patients have no positive wound healing outcome (unhealed wound).
Theoretical and clinical studies prove that the PRP method is a reliable, rational, and modern option for PSW treatment. It should not be seen as the only one and universal.

Keywords: problematic skin wounds, platelet-rich plasma, healing failure,

pdf - Download FULL TEXT /PDF 2338 KB/
Please cite this article as: Sokolov T, Manukova A. Research of Medical Factors and Reasons for Healing Failure of Problematic Skin Wounds Treated with Platelet–Rich Plasma. J of IMAB. 2020 Oct-Dec;26(4):3463-3468. DOI: 10.5272/jimab.2020264.3463

Corresponding AutorCorrespondence to: Dr Tsvetan Sokolov, Department of Orthopedics and Traumatology, UMBAL Kanev Ruse; 2, Nezavisimost str., 7002 Ruse, Bulgaria; E-mail: sokolovi2001@abv.bg

1. Lindholm C, Searle R. Wound management for the 21st century: combining effectiveness and efficiency. Int Wound J. 2016 Jul;13 Suppl 2:5-15. [PubMed]
2. Ashcroft GS, Horan MA, Herrick SE, Tarnuzzer RW, Schultz GS, Ferguson MW. Age-related differences in the temporal and spatial regulation of matrix metalloproteinases (MMPs) in normal skin and acute cutaneous wounds of healthy humans. Cell Tissue Res. 1997 Dec;290(3):581-91. [PubMed]
3. Cancela AM, Lana JF, Annichino-Bizzachi JM, BelangeroWD, Luzo ACM. Use of Platelet-Rich Plasma (PRP) in Treating Chronic Wounds. In: Lana JF, ShimojoAA, Belangero WD, Malheiros Luzo AC. (eds.), Platelet-Rich Plasma. Lecture Notes in Bioengineering. Springer-Verlag Berlin, Heidelberg. 2014; p.281-288. [Crossref]
4. Anitua E, Aguirre JJ, Algorta J, Ayerdi E, Cabezas AI, Orive G, et al. Effectiveness of autologous preparation rich in growth factors for the treatment of chronic cutaneous ulcers. J Biomed Mater Res B Appl Biomater. 2008 Feb;84(2):415-21. [PubMed]
5. Agrawal K, Chauhan N. Pressure ulcers: Back to the basics. Indian J Plast Surg. 2012 May;45(2):244-54. [PubMed]
6. Clark M. Just the Facts. Number 1. Pressure ulcers. Welsh Wound Network. 2010. [Internet]
7.  Sokolov T, Manukova A, Karakoleva S, Valentinov B, Petrova N. Analysis of the results of applying the method platelet-rich plasma (prp) for the treatment of problematic skin wounds. J of IMAB. 2017 Jan-Mar;23(1)1460-1465. [Crossref]
8. Lazarov B, Karakoleva S. Using GAS syntax for education in Mathematics. In: Higher education in Bulgaria and the Europe 2020 strategy. International business school, Botevgrad. IBS Press. 2011; 3:914-921. [Internet]
9. Krupski WC, Reilly LM, Perez S, Moss KM, Crombleholme PA, Rapp JH. A prospective randomized trial of autologous platelet-derived wound healing factors for treatment of chronic nonhealing wounds: a preliminary report. J Vasc Surg. 1991 Oct;14(4):526-32; discussion 532-6. [PubMed]
10. Margolis DJ, Allen-Taylor L, Hoffstad O, Berlin JA. Diabetic neuropathic foot ulcers: the association of wound size, wound duration, and wound grade on healing. Diabetes Care. 2002 Oct;25(10):1835-9. [PubMed]
11. artinez-Zapata MJ, Marti-Carvajal AJ, Sola I, Exposito JA, Bolibar I, Rodriguez L, et al. Autologous platelet-rich plasma for treating chronic wounds. Cochrane Database Syst Rev. 2012 Oct 17;10:CD006899. [PubMed]
12. Martin P, Nunan R. Cellular and molecular mechanisms of repair in acute and chronic wound healing. Br J Dermatol. 2015 May;173(2):370-8. [PubMed]
13. Cheng W, Yan-hua R, Fang-gang N, Guo-an Z.The content and ratio of type I and III collagen in skin differ with age and injury. Afr J Biotechnol. 2011; 10(13):2524-2529.
14. Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstr Surg. 2006 Nov;118(6):147e -159e.
15. Snyder RJ. Treatment of nonhealing ulcers with allografts. Clin Dermatol. 2005 Jul-Aug;23(4):388-95. [PubMed]
16. Jacobson M, Fufa D, Abreu EL, Kevy S, Murray MM. Platelets, but not erythrocytes, significantly affect cytokine release and scaffold contraction in a provisional scaffold model. Wound Repair Regen. 2008 May-Jun;16(3):370-8. [PubMed]
17. Lim CS, Baruah M, Bahia SS. Diagnosis and management of venous leg ulcers. BMJ. 2018 Aug 14;362:k3115. [PubMed]
18. Mizzi A, Cassar K, Bowen C, Formosa C. The progression rate of peripheral arterial disease in patients with intermittent claudication: a systematic review. J Foot Ankle Res. 2019 Aug 6;12:40:1-9. [PubMed]
19.atkov O, Kovachev V, Vasilev V, Kovachev M. [Microvascular surgery in orthopedics and traumatology, plastic and reconstructive surgery - results and prospects.] [in Bulgarian] Angiology and Vascular Surgery. 2015 Oct;18(2):48-50.

Received: 12 November 2019
Published online: 24 November 2020

back to Online Journal