head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2021, vol. 27, issue1
Subject Area: Medicine
DOI: 10.5272/jimab.2021271.3497
Published online: 05 January 2021

Original article

J of IMAB. 2021 Jan-Mar;27(1):3497-3501
Pavlina Andreeva-Gateva1ORCID logo Corresponding Autoremail, Anna Masseva2ORCID logo,
1) Department of Pharmacology and Toxicology, Medical Faculty, Medical University of Sofia, Bulgaria.
2) University Hospital of Obstetrics and Gynecology "Maichin Dom", Bulgaria, Sofia, Bulgaria.

Our study aimed to compare the seroprevalence of anti-Helicobacter pylori antibodies in patients with and without unexplained infertility. Three groups were compared, i.e., patients with idiopathic infertility (Group A, n=105); fertile patients with Helicobacter pylori-associated diseases (Group B, n=105) and fertile subjects lacking H. pylori-related disease (Group C, n=105). Anti-sperm antibodies (ASA) and anti- H. pylori antibodies (AHPA) were evaluated. Similarly high seroprevalence of AHPA in all studied patients was found (p>0.05). Odds ratio calculated against infertility for AHPA-positive patients was 0.97 (95% confidential interval from 0.57 to 1.68, p=0.113). The AHPA-seroprevalence of samples from men was 1.3 - 1.8 times higher than those from women. Also, for ASA-seroprevalence, the men-to-women ratio was up to 2 times higher. We calculated the odds ratio for male and female groups separately and again did not obtain statistical significance in the odds ratios neither for men nor for women. Our findings failed to reveal H. pylori seropositivity as a possible risk factor for idiopathic infertility neither in men nor in women.

Keywords: anti-Helicobacter pylori antibodies, anti-sperm antibodies, idiopathic infertility,

pdf - Download FULL TEXT /PDF 507 KB/
Please cite this article as: Andreeva-Gateva P, Masseva A. Similarly High Seroprevalence of Anti-Helicobacter pylori Antibodies in Men and Women with and without Idiopathic Infertility. J of IMAB. 2021 Jan-Mar;27(1):3497-3501. DOI: 10.5272/jimab.2021271.3497

Corresponding AutorCorrespondence to: Pavlina Andreeva-Gateva, Department of Pharmacology and Toxicology, Medical University - Sofia; 1, G. Sofiiski str., Sofia, Bulgaria; E-mail: pandreeva_gateva@outlook.com

1. ESHRE Capri Workshop Group. Physiopathological determinants of human infertility. Hum Reprod Update. 2002 Sep-Oct;8(5):435-47.  [PubMed] [Crossref]
2. Hossain A, Islam N, Arya S, Madanes A. The prevalence of circulating antisperm antibody (ASA) in the infertile population representing all etiologies. Middle East Fertil Soc J. 2007; 12(1):27-30. [Internet]
3. Chamley LW, Clarke GN. Antisperm antibodies and conception. Semin Immunopathol. 2007 Jun;29(2):169-84. [PubMed] [Crossref]
4. Investigation of fertility problems and management strategies. In: Fertility: Assessment and Treatment for People with Fertility Problems. National Collaborating Centre for Women's and Children's Health (UK). Royal College of Obstetricians and Gynaecologists. February 2013. Chapter 6. pp.80-130. [PubMed]
5. Bohring C, Krause W. Immune infertility: towards a better understanding of sperm (auto) immunity. The value of proteomic analysis. Hum Reprod. 2003 May;18(5):915-24. [PubMed] [Crossref]
6. Rumke P, Hellinga G. Autoantibodies against spermatozoa in sterile men. Am J Clin Pathol. 1959 Oct;32:357-63. [PubMed] [Crossref]
7. Zhang H, Zhao E, Zhang C, Li X. The Change of Semen Superoxide Dismutase and Acrosin Activity in the Sterility of Male Patients with Positive Antisperm Antibody. Cell Biochem Biophys. 2015 Nov;73(2):451-453. [PubMed] [Crossref]
8. Zhao Y, Zhao E, Zhang C, Zhang H. Study of the Changes of Acrosomal Enzyme, Nitric Oxide Synthase, and Superoxide Dismutase of Infertile Patients with Positive Antisperm Antibody in Seminal Plasma. Cell Biochem Biophys. 2015 Dec;73(3):639-42. [PubMed] [Crossref]
9. Cheng CY, Mruk DD. The blood-testis barrier and its implications for male contraception. Pharmacol Rev. 2012 Jan;64(1):16-64. [PubMed] [Crossref]
10. Ambrosini G, Andrisani A, Fiore C, Faggian D, D'Antona D, Ragazzi E, et al. Anti-Helicobacter pylori antibodies in cervical mucus: a new cause of infertility. Eur J Obstet Gynecol Reprod Biol. 2011 Apr;155(2):157-60. [PubMed] [Crossref]
11. Selgrad M, Meyer F, Malfertheiner P. [Helicobacter pylori: short overview on selected data from the history and their value for clinical medicine, in particular, surgery - what does the (general/abdominal) surgeon need to know] [in German] Zentralbl Chir. 2014 Aug;139(4):399-405. [PubMed] [Crossref]
12. Yang SW, Kwon HS, Sohn IS, Kim YJ, Hwang HS. Association of VacA- and Cag A-specific Helicobacter pylori strain infection with spontaneous preterm birth. J Matern Fetal Neonatal Med. 2017 Apr;30(8):995-1000. [PubMed] [Crossref]
13. Sobala GM, Crabtree JE, Dixon MF, Schorah CJ, Taylor JD, Rathbone BJ, et al. Acute Helicobacter pylori infection: clinical features, local and systemic immune response, gastric mucosal histology, and gastric juice ascorbic acid concentrations. Gut. 1991 Nov;32(11):1415-8. [PubMed] [Crossref]
14. Marietti M, Gasbarrini A, Saracco G, Pellicano R. Helicobacter pylori infection and diabetes mellitus: the 2013 state of the art. Panminerva Med. 2013 Sep;55(3):277-81. [PubMed]
15. Figura N, Piomboni P, Ponzetto A, Gambera L, Lenzi C, Vaira D, et al. Helicobacter pylori infection and infertility. Eur J Gastroenterol Hepatol. 2002  Jun;14(6):663-9. [PubMed] [Crossref]
16. El-Garem Y, El-Sawy M, Mostafa T. Seminal Helicobacter pylori treatment improves sperm motility in infertile asthenozoospermic men. Urology. 2014  Dec;84(6):1347-50. [PubMed] [Crossref]
17. Caviglia GP, Fagoonee S, Pellicano R. Re: El-Garem et al.: Seminal Helicobacter pylori Treatment Improves Sperm Motility in Infertile Asthenozoospermic Men (Urology 2014;84:1347-1350). Urology 2015  May;85(5):1217. [PubMed] [Crossref]
18. Yasin AL, Yasin AL, Basha WS. The Epidemiology of Anti-Sperm Antibodies Among Couples with Unexplained Infertility in North West Bank, Palestine. J Clin Diagn Res. 2016 Mar;10(3):QC01-3. [PubMed] [Crossref]
19. Fu J, Yao R, Luo Y, Yang D, Cao Y, Qiu Y, et al. Immune Infertility Should Be Positively Diagnosed Using an Accurate Method by Monitoring the Level of Anti-ACTL7a Antibody. Sci Rep. 2016  Mar 9;6:22844. [PubMed] [Crossref]
20. Friberg J. A simple and sensitive micro-method for demonstration of sperm-agglutinating activity in serum from infertile men and women. Acta Obstet Gynecol Scand Suppl. 1974; 36:21-9. [PubMed] [Crossref].

Received: 11 February 2020
Published online: 05 January 2021

back to Online Journal