 |
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2021, vol. 27, issue1
Subject Area: Medicine
-
DOI: 10.5272/jimab.2021271.3506
Published online: 07 January 2021
Original article
J of IMAB. 2021 Jan-Mar;27(1):3506-3509
ANTIBACTERIAL ACTIVITY OF DIFFERENT EXTRACTS OF BLACK MUSSEL (MYTILUS GALLOPROVINCIALIS) FROM THE BLACK SEA, BULGARIA
Gabriela Tsankova1
, Tatina Todorova1, Neli Ermenlieva1, Albena Merdzhanova2, Veselina Panayotova2, Diana Dobreva2, Katya Peytcheva2,
1) Department of Microbiology and Virology, Faculty of Medicine, Medical University of Varna, Bulgaria.
2) Department of Chemistry, Faculty of Pharmacy, Medical University of Varna, Bulgaria.
ABSTRACT:
Background: Over the past decade, there has been a growing interest in sea bivalves, which are an inexpensive and easily accessible source of high-quality proteins, lipids and secondary metabolites with antimicrobial and anti-fungal potential. Farmed Black Sea mussel (M. galloprovincialis) are promising objects for the study of their antimicrobial potential.
Purpose: The aim of this work is to determine the antibacterial activity of different extracts from the Black Sea mussel Mytilus galloprovincialis tissues by using the disc diffusion method with cultures of Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae.
Material/Methods: Extraction of mussel tissues was done with different solvents: ethyl acetate (100%), methanol (100%), glycerol:water (50%, 1:1 v/v), ethanol (50%), acetone (70%), hot water. Antimicrobial activities of these extracts from Mytilus galloprovincialis was assessed by the disc-diffusion method.
Results: Testing antibacterial activity of black mussels revealed that ethyl acetate extract showed the highest activity against Еscherichia coli (13 mm) and Klebsiella pneumoniae (11 mm) and no activity against Staphilococcus aureus. The glycerol: water extract showed growth inhibition effect against Staphylococcus aureus (11 mm) and Escherichia coli (10 mm), but no effect against Klebsiella pneumoniae.
Conclusions: The preliminary information presented in this study showed that the Black Sea farmed mussel could be an interesting source of antibacterial compounds. The glycerol-water extracts of Mytilus galloprovincialis had low antimicrobial activities against Staphylococcus aureus and more important against Escherichia coli.
Keywords: antibacterial activity, Mytilus galloprovincialis, disc-diffusion method,
- Download FULL TEXT /PDF 781 KB/
Please cite this article as: Tsankova G, Todorova T, Ermenlieva N, Merdzhanova A, Panayotova V, Dobreva D, Peytcheva K. Antibacterial activity of different extracts of black mussel (Mytilus galloprovincialis) from the Black Sea, Bulgaria. J of IMAB. 2021 Jan-Mar;27(1):3506-3509. DOI: 10.5272/jimab.2021271.3506
Correspondence to: Gabriela Tsankova, Department of Microbiology and Virology, Faculty of Medicine, Medical University of Varna; 3, Bregalniza Str., 9002 Varna, Bulgaria; E-mail: gabriela_sc@abv.bg
REFERENCES:
1. Annual report on the state and development of agriculture (Agricultural report ‘2019). Ministry of Agriculture, Food and Forestry. Bulgaria. 2019. [Internet]
2. Donia M, Hamann MT. Marine natural products and their potential applications as anti-infective agents. Lancet Infect Dis. 2003 Jun;3(6):338-48. [PubMed]
3. Rуїaсska H, Michalski M, Osek J. Antibacterial activity of tissues of bivalve molluscs available on Polish market. Bull Vet Inst Pulawy. 2012 Dec 1;56(4):569–71. [Crossref]
4. Haug T, Stensvag K, Olsen M OM, Sandsdalen E, Styrvold OB. Antibacterial activities in various tissues of the horse mussel, Modiolus modiolus. J Invertebr Pathol. 2004 Feb;85(2):112-9. [PubMed]
5. Grienke U, Silke J, Tasdemir D. Bioactive compounds from marine mussels and their effects on human health. Food Chem. 2014 Jan 1;142:48–60. [PubMed]
6. Anitha C, Basil-Rose MR. Antimicrobial activity of the hemolymph and whole body extract of a frech water clam, Villorita cyprinoides (Gray, 1825). Int J Zool Appl Biosci. 2018 Feb 1;3(2):151-6. [Crossref]
7. Ky PX, Mien PT, Hy LHK, Ha DV, Anh NP, Thiet DT, et al. Investigation of antibacterial activity of crude extracts from marine snails and bivalves in the Southern coast of Vietnam. Am J Biomed Life Sci. 2019 Apr 10;7(1):10-15. [Crossref]
8. Shan B, Cai Y-Z, Brooks JD, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int J Food Microbiol. 2007 Jun 10;117(1):112–9. [PubMed]
9. Kiran N, Siddiqui G, Khan A, Ibrar K, Tushar, P. Extraction and screening of bioactive compounds with antimicrobial properties from selected species of mollusk and crustacean. J Clin Cell Immunol. 2014; 5(1):1000189. [Crossref]
10. Nightingale S, Mettilda S, Vinoliya J. Antimicrobial activity of whole body extract of slug Mariaella dussumieri (Gray, 1855) against clinical pathogens. J Glob Trends Pharm Sci. 2018;9(1):4917-25.
11. Badiu DL, Balu AM, Barbes L, Luque R, Nita R, Radu M, et al. Physico-chemical characterization of lipids from Mytilus galloprovincialis (L.) and Rapana venosa and their healing properties on skin burns. Lipids. 2008 Sep 10;43(9):829-41. [PubMed].
Received: 28 February 2020
Published online: 07 January 2021