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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2021, vol. 27, issue2
Subject Area: Dental Medicine
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DOI: 10.5272/jimab.2021272.3692
Published online: 07 April 2021
Original article
J of IMAB. 2021 Apr-Jun;27(2):3692-3699
PRESENCE OF SNP OF INTERLEUKIN17F IN PATIENTS WITH PERIODONTITIS IN A BULGARIAN POPULATION
Antoaneta Mlachkova
, Zdravka Pashova-Tasseva 
, Christina Popova, Maya Kicheva,
Department of Periodontology, Faculty of Dental Medicine, Medical University - Sofia, Bulgaria.
ABSTRACT:
Introduction: It`s well known that the periodontitis is а complex disease initiated by bacteria, but modified by environmental factors and the host response. As any other chronic disease, the periodontal disease is influenced by the individual predisposition of the patient to develop the specific symptoms. The increasing number of studies in the area of the genetic factors and mechanisms of the patient to develop the specific condition is leading to the need for certain polymоrphism’s to be studied in details for the Bulgarian population.
Aim: The recent study aims to identify the presence of SNP of IL-17F in the Bulgarian population.
Materials and methods: In the study, 40 patients with periodontitis stage II, III and IV and 10 healthy control subjects were taking part. The age of the subjects varied between 23 and 75 with an average value of 46 years. Clinical and radiographic methods to establish the basic periodontal parameters were used. Laboratory methods were performed by means of Real-Time PCR for determination of SNP of Interleukin 17F (IL-17F) (-7488C/T rs_763780). The statistic data was processed with PCA – IBM SPSS Statistics Version 21. From all of the patients, informed consent was taken.
Results: The recent study collected information about the dominating genotype when studying SNP of IL-17 F for patients with periodontitis. The presence of two genotypes was established – genotype TT (92%) and genotype CT (8%). We have established specific tendencies about the distribution of major parameter for diagnosis of periodontitis such as BoP and BL/Age in both groups. The individual host susceptibility can be used as a diagnostic parameter leading to the development of screening methods in order suspectable individuals to be found.
Conclusion: The study has contributed to clarifying the genetic characteristic of the tested subject. The results confirmed the data from different studies that aim to research the genetic polymorphism of IL-17F in relation to periodontitis.
Keywords: periodontitis, gene polymorphism, SNP, Interleukin-17F,
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Please cite this article as: Mlachkova A, Pashova-Tasseva Z, Popova H, Kicheva M. Presence of SNP of Interleukin17F in patients with periodontitis in a Bulgarian population. J of IMAB. 2021 Apr-Jun;27(1):3692-3699. DOI: 10.5272/jimab.2021272.3692
Correspondence to: Zdavka Pashova-Tasseva, Department of Periodontology, Faculty of Dental Medicine, Medical University - Sofia; 1, St. George Sofiiski str., 1431 Sofia, Bulgaria; E-mail: z.pashova@abv.bg
REFERENCES:
1. Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol. 2018 Jun;89 Suppl 1:S173-S182. [PubMed]
2. Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol. 2018 Jun;89 Suppl 1:S159-S172. [PubMed]
3. Hart TC, Kornman KS. Genetic factors in the pathogenesis of periodontitis. Periodontol 2000. 1997 Jun;14:202-15. [PubMed]
4. Laine ML, Crielaard W, Loos BG. Genetic susceptibility to periodontitis. Periodontol 2000. 2012 Feb;58(1):37-68. [PubMed]
5. Tâlvan T, Mohor C, Chisnoiu D, Cristea V, Câmpian S. Expression of Interleukin (IL)-1β, IL-8, IL-10 and IL-13 in chronic adult periodontitis progression. Arch Med. 2017; 9:3-11.
6. Larsson L, Thorbert-Mros S, Rymo L, Berglundh T. Interleukin-10 Genotypes of the -1087 Single Nucleotide Polymorphism Influence Sp1 Expression in Periodontitis Lesions. J Periodontol. 2011 Sep;82(9):1376-82. [PubMed]
7. Nicu EA, Loos BG. Polymorphonuclear neutrophils in periodontitis and their possible modulation as a therapeutic approach. Periodontol 2000. 2016 Jun;71(1):140-63. [PubMed]
8. Persson GR. Dental geriatrics and periodontitis. Periodontol 2000. 2017 Jun;74(1):102-115. [PubMed]
9. Taylor JJ, Preshaw PM, Donaldson PT. Cytokine gene polymorphism and immunoregulation in periodontal disease. Periodontol 2000. 2004; 35:158-82. [PubMed]
10. Dosseva-Panova V. [Bacterial findings and inflammatory components of the host response in severe chronic periodontitis.] [dissertation]. Sofia: Medical University- Sofia; 2018. 217 p. [in Bulgarian]
11. Mlachkova A, Popova Hr, Kicheva M. Severity of chronic periodontitis in relation with IL-1B gene polymorphism. Problems of Dental Medicine. 2011; 37(1):6-14
12. Chen X, Huang J, Zhong L, Ding C. Quantitative assessment of the associations between interleukin-8 polymorphisms and periodontitis susceptibility. J Periodontol. 2015 Feb;86(2):292-300. [PubMed]
13. Chen D, Zhang T-L, Wang X. Association between Polymorphisms in Interleukins 4 and 13 Genes and Chronic Periodontitis in a Han Chinese Population. Biomed Res Int. 2016;2016:8389020. [PubMed]
14. Popova Chr, Dosseva-Panova V, Mlachkova A. Correlation of gene expression of main inflammatory cytokines and the severity of chronic periodontitis. J of IMAB. 2014 Jul-Sep;20(3):563-566. [Crossref]
15. Schaefer AS. Genetics of periodontitis: Discovery, biology, and clinical impact. Periodontol 2000. 2018 Oct;78(1):162-173. [PubMed]
16. Lester SR, Bain JL, Johnson RB, Serio FG. Gingival concentrations of interleukin-23 and -17 at healthy sites and at sites of clinical attachment loss. J Periodontol. 2007 Aug;78(8):1545-50. [PubMed]
17. Yoshie H, Kobayashi T, Tai H, Galicia JC. The role of genetic polymorphisms in periodontitis. Periodontol 2000. 2007; 43:102-32. [PubMed]
18. Zacarias JM, Sippert EA, Tsuneto PY, Visentainer JE, de Oliveira e Silva C, Sell AM. The Influence of Interleukin 17A and IL17F Polymorphisms on Chronic Periodontitis Disease in Brazilian Patients. Mediators Inflamm. 2015;2015:147056. [PubMed]
19. Zhang J, Sun X, Xiao L, Xie C, Xuan D, Luo G. Gene polymorphisms and periodontitis. Periodontol 2000. 2011 Jun;56(1):102-24. [PubMed]
20. Erdemir E, Hendek K, Kocakap B, Ozkan Y. Interleukin (IL)-17F and IL-23-R gene polymorphisms in Turkish population with periodontitis. J Res Med Den Sci. 2015 Apr-Jun;3(2):104-8.
21. Laine ML, Loos BG, Crielaard W. Gene polymorphisms in chronic periodontitis. Int J Dent. 2010;2010:324719. [PubMed]
22. Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al.Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015 May-Jun;23(3):329-55. [PubMed]
23. Mazurek-Mochol M, Kozak M, Malinowski D, Safranow K, Pawlik A. IL-17F Gene rs763780 and IL-17A rs2275913 Polymorphismsin Patients with Periodontitis. Int J Environ Res Public Health. 2021 Jan 26;18(3):1081. [PubMed].
Received: 13 April 2020
Published online: 07 April 2021