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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2021, vol. 27, issue4
Subject Area: Medicine
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DOI: 10.5272/jimab.2021274.4035
Published online: 13 October 2021
Original article
J of IMAB. 2021 Oct-Dec;27(4):4035-4037
TWO ALTERNATIVE METHODS FOR ANAEROBIC CULTIVATION OF BACTEROIDES FRAGILIS ATCC 25285 COMPARED TO THE EFFICIENCY OF CULTIVATION IN A GAS PACK SYSTEM
Neli M. Ermenlieva1
, Emilia P. Georgieva2, Sylvia Y. Stamova3, Svetlana F. Georgieva3, Silvia G. Mihaylova4,
1) Department of Microbiology and Virology, Faculty of Medicine, Medical University – Varna, Bulgaria.
2) TRS Medical laboratory assistant, Medical College, Medical University-Varna, Bulgaria.
3) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University – Varna, Bulgaria.
4) TRS Assistant Pharmacist, Medical College, Medical University of Varna, Bulgaria.
ABSTRACT:
Cultivation of anaerobes is not a routine procedure. In most cases, it is carried out in reference laboratories with the help of special equipment, including an anaerobic chamber or anaerobic jar with Gas pack system. There are some older methods so-called the Candlejar system and the Fortner principle.
The aim of the study is to compare two alternative methods for anaerobic cultivation of Bacteroides fragilis ATCC 25285 with cultivation by Gas Pack system, which is a widely used and well studied method.
We made anaerobic cultivation using the GasPack system, Candle jar and Fortner principle. The bacteria were seed in two different types of agar media - suitable for anaerobic cultivation Wilkins-Chalgren agar and Mueller-Hinton agar, useful for antibiotic sensitivity testing.
The results of our study showed that in all bacterial cultures, B. fragilis ATCC 25285 demonstrated heavy growth. The Candle jar system and Fortner principle were found to be a sensitive and cost-effective alternative that might be used in resource-limited settings.
Keywords: B. fragilis, cultivation, Fortner principle, Candle jar, alternative methods,
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Please cite this article as: Ermenlieva NM, Georgieva EP, Stamova SY, Georgieva SF, Mihaylova SG. Two alternative methods for anaerobic cultivation of Bacteroides fragilis ATCC 25285 compared to the efficiency of cultivation in a Gas Pack system. J of IMAB. 2021 Oct-Dec;27(4):4035-4037. DOI: 10.5272/jimab.2021274.4035
Correspondence to: Neli Mitkova Ermenlieva, Department of Microbiology and Virology, Faculty of Medicine, Medical University – Varna; 3, Bregalniza Str., 9002 Varna, Bulgaria; E-mail: n.ermenlieva@abv.bg
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Received: 08 March 2021
Published online: 13 October 2021