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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2021, vol. 27, issue4
Subject Area: Medicine
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DOI: 10.5272/jimab.2021274.4143
Published online: 09 December 2021
Original article
J of IMAB. 2021 Oct-Dec;27(4):4143-4150
EFFECT OF OLIGOSACCHARIDES ON ENZYMES OF CARBOHYDRATE METABOLISM AND ANTIOXIDANT PROTECTION IN IN VITRO TREATED ERYTHROCYTES UNDER CONDITIONS OF HYPERGLYCEMIA
Ivica Dimov1
, Mariya Choneva1, Ilia lliev2, Anelia Bivolarska1,
1) Department of Biochemistry, Faculty of Pharmacy, Medical University of Plovdiv, Bulgaria.
2) Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University "Paisii Hilendarski", Bulgaria.
ABSTRACT:
Introduction: The purpose of this experiment is to examine the effect of different oligosaccharides with proven prebiotic effects on enzymes of carbohydrate metabolism and the antioxidant protection of erythrocytes in vitro under conditions of hyperglycemia.
Materials and methods: This experiment included 10 healthy men (27±3 years of age). The isolated erythrocytes were treated with 1% and 5% solutions of the following oligosaccharides: lactulose, inulin, galactooligosaccharide and fructooligosaccharide in the presence of 5mM, 50mM and 100mM glucose. After incubation, for 2 hours at 37 °C, the erythrocytes were lysed, and the supernatant was used for analyses of lactate dehydrogenase, hexokinase and glutathione reductase. FRAP (Ferric reducing antioxidant power) method was used for determining the total antioxidant activity of erythrocytes.
Results: Lactate dehydrogenase was decreased in the presence of 5% lactulose in groups with 50mM and 100 mM Glc. An increase in the activity of glutathione reductase under severe hyperglycemia (100mM glucose) was observed after treatment with: 1% lactulose, 1% inulin, 1% galactooligosaccharide, 1% and 5% fructooligosaccharides (p<0.005). A significant difference in the enzymatic activity of hexokinase was found in all groups (p<0.05) and of glutathione reductase only in the control group as well as in the groups treated with 1% lactulose, 1% galactooligosaccharide, 1% and 5% fructooligosaccharides
Conclusions: Galactooligosaccharides 1% and fructooligosaccharides 1% and 5% cause a statistically significant increase of the enzymatic activities of hexokinase and glutathione reductase in in vitro hyperglycemia induced by 100 mM glucose, as well as an increase in FRAP.
Keywords: prebiotics, oligosaccharides, hyperglycemia, erythrocytes, antioxidant protection,
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Please cite this article as: Dimov I, Choneva M, lliev I, Bivolarska A. Effect of oligosaccharides on enzymes of carbohydrate metabolism and antioxidant protection in in vitro treated erythrocytes under conditions of hyperglycemia. J of IMAB. 2021 Oct-Dec;27(4):4143-4150.
DOI: 10.5272/jimab.2021274.4143
Correspondence to: Asst. Prof. Ivica Dimov, Department of Biochemistry, Faculty of Pharmacy, Medical University of Plovdiv; 15-A, Vasil Aprilov Blvd., Plovdiv 4002, Bulgaria; E-mail: ivicadimov@gmail.com
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Received: 20 January 2021
Published online: 09 December 2021