 |
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2022, vol. 28, issue4
Subject Area: Dental Medicine
-
DOI: 10.5272/jimab.2022284.4711
Published online: 29 November 2022
Original article
J of IMAB. 2022 Oct-Dec;28(4):4711-4714
RAMAN SPECTROSCOPY ANALYSIS OF CORONARY DENTIN OF VITAL AND ENDODONTICALLY TREATED TEETH
Ekaterina Karteva1
, Neshka Manchorova1, Teodora Karteva1, Zhelyazko Damyanov2,
1) Operative Dentistry and Endodontics, Faculty of Dental Medicine, Medical University, Plovdiv, Bulgaria.
2) Institute of Mineralogy and Crystallography, Bulgarian Academy of Sciences, Sofia, Bulgaria.
ABSTRACT:
PURPOSE: The present study aimed to investigate the properties of coronary dentin of vital and endodontically treated human teeth using Raman Spectroscopy.
MATERIAL/METHODS: The samples used were taken from coronary dentin of intact extracted human teeth, ultrasonically cleaned and stored in a 0.2% thymol solution at 4 degrees Celsius for up to 3 months. They were divided into two groups – “vital dentin” and “endodontically treated dentin” samples. Each sample was crossed-sectioned, embedded in resin, polished and subjected to Raman spectroscopy with a Horiba Jobin-Yvon T64000 triple-grating spectrometer, using a CdHe laser with 4 μm diameter of the laser spot and 2.5mW of power on the examined surface. The resultant diagrams were analyzed using the software package Origin 9.6 andunderwent baseline correction and normalization. A comparison between the two studied groups was made.
RESULTS: The results from the “vital dentin” group showed a well-expressed crystalline structure, with clear peaks corresponding to pure and b-carbonated hydroxyapatite. The results of the “endodontically treated dentin” group showed the presence of new chemical compounds – octacalcium phosphate, tricalcium phosphate and dicalcium phosphate dihydrate, as well as increased intensity of the b-carbonated hydroxyapatite peaks.
CONCLUSIONS: The presence of mineral substances with low calcium content could contribute to a decrease in dentin toughness, which in turn can lessen the mechanical properties of endodontically treated teeth. Raman spectroscopy can be a useful tool in the detection of these dentin components.
Keywords: Raman spectroscopy, endodontically treated teeth, crown dentin,
- Download FULL TEXT /PDF 617 KB/
Please cite this article as: Karteva E, Manchorova N, Karteva T, Damyanov Z. Raman spectroscopy analysis of coronary dentin of vital and endodontically treated teeth. J of IMAB. 2022 Oct-Dec;28(4):4711-4714. DOI: 10.5272/jimab.2022284.4711
Correspondence to: Ekaterina Karteva, Department of Operative dentistry and endodontics,Faculty of dental medicine, Medical University- Plovdiv; 3, Hristo Botev Blvd., Plovdiv, Bulgaria; E-mail: ekaterina.karteva@mu-plovdiv.bg
REFERENCES:
1. Esmonde-White KA, Cuellar M, Lewis IR. The role of Raman spectroscopy in biopharmaceuticals from development to manufacturing. Anal Bioanal Chem. 2021 Jan;414(2):969-991. [PubMed]
2. Auner GW, Koya SK, Huang C, Broadbent B, Trexler M, Auner Z et al. Applications of Raman spectroscopy in cancer diagnosis. Cancer Metastasis Rev. 2018 Dec;37(4):691-717. [PubMed]
3. DevittG, Howard K, Mudher A, MahajanS. Raman spectroscopy: an emerging tool in neurodegenerative disease researchand diagnosis. ACS Chem. Neurosci. 2018 Mar 21;9(3):404-420. [PubMed]
4. Kinney JH, Nalla RK, Pople JA, Breunig TM, Ritchie RO. Age-related transparent root dentin: mineral concentration, crystallite size, and mechanical properties. Biomaterials 2005 Jun;26(16):3363–3376. [PubMed]
5.Yan W, Montoya C, Øilo M, Ossa A, Paranjpe A, Zhang H et al. Reduction in fracture resistance of the root with ageing. ;J Endod. 2017 Sep;43(9):1494-1498. [PubMed]
6. Penel G, Leroy G, Rey C, Bres E. MicroRaman spectral study of the PO4 and CO3 vibrational modes in synthetic and biological apatites. Calcif Tissue Int.1998 Dec;63(6):475-481. [PubMed]
7. Koutsopoulos, S. Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods. J Biomed Mater. 2002 Dec;62(4):600-612. [PubMed]
8. Dorozhkin SV. Chapter 7: Nanodimensional and nanocrystalline calcium orthophosphates. In: Frontiers in nanobiomedical research. Tissue regeneration. World Scientific. 2014: 219-341 [Crossref]
9. Rey C, Combes C, Drouet C, Sfihi H, Barroug A. Physico-chemical properties of nanocrystalline apatites: implications for biominerals and biomaterials. Mater Sci Eng: C. 2007 Mar;27(2):198-205. [Crossref]
10. Eichert D, Drouet C, Sfihi H, Rey C, Combes C. Nanocrystalline apatite-based biomaterials: synthesis, processing and characterization. In: Biomaterials Research Advances. 2008: 93-143. [Internet]
11. Soares CJ, Santana FR, Silva NR, Preira JC, Pereira CA. Influence of the endodontic treatment on mechanical properties of root dentin. J. Endod. 2007 May;33(5):603–606. [PubMed]
12. Kishen A, Asundi A. Experimental investigation on the role of water in the mechanical behavior of structural dentine. J. Biomed. Mater. Res. Part A. 2005 May;73(2):192–200. . [PubMed]
13.Yan W, Jiang H, Deng Z, Paranjpe A, Zhang H, Arola D. Shrinkage Strains in the Dentin of Endodontically Treated Teeth with Water Loss. J Endod. 2021 May;47(5):806-811. [PubMed]
14. Zhang D, Mao S, Lu C, Romberg E, Arola D. Dehydration and the dynamic dimensional changes within dentin and enamel. Dent. Mater. 2009 July;25(7):937–945. [PubMed]
15. Suchanek W, Yoshimura M. Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants. J Mater Res. 1998 Jan;13(1)94-117. [Crossref]
16. Suzuki O, Shiwaku Y, Hamai R. Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials. Dent Mater. 2020 Mar;39(2):187-199.
17. Yokoi T, Shimabukuro M, Kawashita M. Octacalcium phosphate with incorporated carboxylate ions: a review. Sci Technol Adv Mater. 2022 Jul;23(1):434-445. [PubMed]
18. Charriere E, Terrazzoni S, Pittet C, Mordasini PH, Dutoit M, Lemaitre J, et al. Mechanical characterization of brushite and hydroxyapatitecements. Biomaterials. 2001 Nov;22(21), 2937-2945. [PubMed]
19. Johnson AJW, Herschler BA. A review of the mechanical behavior of CaP and CaP/polymer composites for applications in bone replacement and repair. Acta Biomater. 2011 Jan;7(1):16-30. [PubMed ]
20. Toledano-Osorio M, Aguilera FS, Osorio R, Muñoz-Soto E, Pérez-Álvarez MC, López-López MT, et al. Hydroxyapatite-based cements induce different apatite formation in radicular dentin. Dent Mater. 2020 Jan;36(1), 167–178. [PubMed]
21. Toledano M, Osorio R, Vallecillo-Rivas M, Osorio E, Lynch CD, Aguilera FS, et al. Zn-doping of silicate and hydroxyapatite-based cements: Dentin mechanobiology and bioactivity. J Mech Behav Biomed Mater.2021 Feb;114:104232. [PubMed]
Received: 05 April 2022
Published online: 29 November 2022