head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2022, vol. 28, issue4
Subject Area: Dental Medicine
DOI: 10.5272/jimab.2022284.4711
Published online: 29 November 2022

Original article
J of IMAB. 2022 Oct-Dec;28(4):4711-4714
Ekaterina Karteva1ORCID logo Corresponding Autoremail, Neshka Manchorova1ORCID logo, Teodora Karteva1ORCID logo, Zhelyazko Damyanov2ORCID logo,
1) Operative Dentistry and Endodontics, Faculty of Dental Medicine, Medical University, Plovdiv, Bulgaria.
2) Institute of Mineralogy and Crystallography, Bulgarian Academy of Sciences, Sofia, Bulgaria.

PURPOSE: The present study aimed to investigate the properties of coronary dentin of vital and endodontically treated human teeth using Raman Spectroscopy.
MATERIAL/METHODS: The samples used were taken from coronary dentin of intact extracted human teeth, ultrasonically cleaned and stored in a 0.2% thymol solution at 4 degrees Celsius for up to 3 months. They were divided into two groups – “vital dentin” and “endodontically treated dentin” samples. Each sample was crossed-sectioned, embedded in resin, polished and subjected to Raman spectroscopy with a Horiba Jobin-Yvon T64000 triple-grating spectrometer, using a CdHe laser with 4 μm diameter of the laser spot and 2.5mW of power on the examined surface. The resultant diagrams were analyzed using the software package Origin 9.6 andunderwent baseline correction and normalization. A comparison between the two studied groups was made.
RESULTS: The results from the “vital dentin” group showed a well-expressed crystalline structure, with clear peaks corresponding to pure and b-carbonated hydroxyapatite. The results of the “endodontically treated dentin” group showed the presence of new chemical compounds – octacalcium phosphate, tricalcium phosphate and dicalcium phosphate dihydrate, as well as increased intensity of the b-carbonated hydroxyapatite peaks.
CONCLUSIONS: The presence of mineral substances with low calcium content could contribute to a decrease in dentin toughness, which in turn can lessen the mechanical properties of endodontically treated teeth. Raman spectroscopy can be a useful tool in the detection of these dentin components.

Keywords: Raman spectroscopy, endodontically treated teeth, crown dentin,

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Please cite this article as: Karteva E, Manchorova N, Karteva T, Damyanov Z. Raman spectroscopy analysis of coronary dentin of vital and endodontically treated teeth. J of IMAB. 2022 Oct-Dec;28(4):4711-4714. DOI: 10.5272/jimab.2022284.4711

Corresponding AutorCorrespondence to: Ekaterina Karteva, Department of Operative dentistry and endodontics,Faculty of dental medicine, Medical University- Plovdiv; 3, Hristo Botev Blvd., Plovdiv, Bulgaria; E-mail: ekaterina.karteva@mu-plovdiv.bg

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Received: 05 April 2022
Published online: 29 November 2022

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