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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2025, vol. 31, issue4
Subject Area: Medicine
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DOI: 10.5272/jimab.2025314.6604
Published online: 13 November 2025
Original article
J of IMAB. 2025 Oct-Dec;31(4):6604-6608
THE CORRELATION BETWEEN NITRIC OXIDE RADICALS AND IL-1Β PROTEIN IN NEWLY DIAGNOSED DIABETES MELLITUS TYPE 2
Petya Goycheva
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Department of Propaedeutics of Internal medicine, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria.
ABSTRACT:
Throughout life, the human body encounters numerous environmental and metabolic factors that generate reactive oxygen and nitrogen species (ROS/RNS). These reactive molecules can cause significant oxidative damage to cellular components. To maintain physiological balance, the body employs sophisticated endogenous antioxidant defense systems that neutralize these harmful free radicals and protect cellular integrity. This investigation sought to 1) examine the fundamental mechanisms of oxidative stress (OS) in diabetes mellitus type 2 (T2DM); 2) analyze the relationship between nitric oxide (NO) radicals and IL-1β protein levels in recently diagnosed T2DM cases; and 3) compare the OS biomarkers between newly diagnosed and long-standing T2DM. The study enrolled 62 patients stratified into the long-standing T2DM group (n=42; disease duration >5 years) and the newly diagnosed T2DM group (n=20; diagnosis within the past year). All results were compared with healthy control subjects (n=33). Interleukin-1 beta (IL-1β) protein concentrations were significantly elevated in newly diagnosed T2DM compared to controls (p < 0.001) and long-standing T2DM (p < 0.001). NO levels showed marked increases in recent-onset diabetics relative to the controls (p < 0.001) and long-standing diabetes patients (p < 0.001).
The study reveals that OS markers and inflammatory cytokines are already substantially elevated at the time of diabetes diagnosis. The pathophysiological processes underlying diabetes begin well before clinical manifestation. The concept of glycemic memory may explain these early biochemical changes. The OS biomarkers could serve as valuable early detection tools for prediabetic states. These findings highlight the importance of early OS-monitoring in at-risk populations and potential therapeutic interventions targeting inflammatory pathways.
Keywords: NO radicals, type 2 diabetes mellitus, IL-1β protein,
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Please cite this article as: Goycheva P. The correlation between Nitric Oxide radicals and IL-1b protein in newly diagnosed diabetes mellitus Type 2. J of IMAB. 2025 Oct-Dec;31(4):6604-6608. [Crossref - 10.5272/jimab.2025314.6604]
Correspondence to: Petya Goycheva, Propaedeutic of Internal Diseases Department, Medical Faculty, Trakia University Hospital; Vazrazhdane district, 2, General Stoletov Str., 6003 Stara Zagora, Bulgaria; E-mail: petya.goycheva@trakia-uni.bg
REFERENCES:
1. Lima JEBF, Moreira NCS, Sakamoto-Hojo ET. Mechanisms underlying the pathophysiology of type 2 diabetes: From risk factors to oxidative stress, metabolic dysfunction, and hyperglycemia. Mutat Res Genet Toxicol Environ Mutagen. 2022 Feb-Mar;874-875:503437. [PubMed]
2.Rahman MS, Hossain KS, Das S, Kundu S, Adegoke EO, Rahman MA, et al. Role of Insulin in Health and Disease: An Update. Intern J Mol Sci 2021 Jun 15;22(12):6403. [PubMed]
3. Cignarelli A, Genchi VA, Perrini S, Natalicchio A, Laviola L, Giorgino F. Insulin and Insulin Receptors in Adipose Tissue Development. Intern J Mol Sci 2019 Feb 11;20(3):759. [PubMed]
4. Pancheva R, Dolinsek J, Panayotova M, Yankov I, Kofinova D, Nikolova S, et al. Bridging the Gap: Awareness, Knowledge, and Challenges of Living with Celiac Disease in Bulgaria. Nutrients. 2025 Apr 4;17(7):1267. [PubMed]
5.Galicia-Garcia U, Benito-Vicente A, Jebari S, Larrea-Sebal A, Siddiqi H, Uribe KB, et al. Pathophysiology of Type 2 Diabetes Mellitus. Int J Mol Sci 2020 Aug 30;21(17):6275. [PubMed]
6.Shi H, Sui Y, Wang X, Luo Y, Ji L. Hydroxyl radical production and oxidative damage induced by cadmium and naphthalene in liver of Carassius auratus. Comp. Biochem. Physiol. Part C Toxicol. Pharmacol. 2005 Jan;140(1):115-21. [PubMed]
7.Yokoyama K, Hashiba K, Wakabayashi H, Hashimoto K, Satoh K, Kurihara T, et al. Inhibition of LPS-stimulated NO production in mouse macrophage-like cells by tropolones. Anticancer Res. 2004 Nov-Dec;24(6):3917-22. [PubMed]
8. Yoshioka T, Iwamoto N, Ito K. An application of electron paramagnetic resonance to evaluate nitric oxide and its quenchers. J. Am. Soc. Nephrol. 1996 Jun;7(6):961-5. [PubMed]
9. Kim ME, Lee JS. Advances in the Regulation of Inflammatory Mediators in Nitric Oxide Synthase: Implications for Disease Modulation and Therapeutic Approaches. Int J Mol Sci 2025 Jan 30;26(3):1204. [PubMed]
10. Blanco S. Muñoz-Gallardo M, Hernández R. Peinado Á. The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology. Antioxidants (Basel). 2025 Jun 13;14(6):724. 2025, 14, 724. [PubMed]
11. Stanimirovic J, Radovanovic J, Banjac K, Obradovic M, Essack M, Zafirovic S, et al. Role of C-Reactive Protein in Diabetic Inflammation. Mediators Inflamm 2022 May 17;2022:3706508. [PubMed]
12. Panayotova M, Penkova M. Measurement of oxidative stress-related markers in gastro-intestinal damages in Bulgarian pediatric patients. Bulg Chem Communic 2024; 142.
13. Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, et al. Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans. Clinical chemistry, 2007 Jan;53(1):34-41. [PubMed]
14. Srikanthan K, Feyh A, Visweshwar H, Shapiro JI, Sodhi K. Systematic Review of Metabolic Syndrome Biomarkers: A Panel for Early Detection, Management, and Risk Stratification in the West Virginian Population. Int J Med Sci 2016 Jan 1;13(1):25-38. [PubMed]
15. Rohm TV, Meier DT, Olefsky JM, Donath MY. Inflammation in obesity, diabetes, and related disorders. Immunity, 2022 Jan 11;55(1):31-55. [PubMed]
Received: 07 July 2025
Published online: 13 November 2025