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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2025, vol. 31, issue4
Subject Area: Medicine
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DOI: 10.5272/jimab.2025314.6680
Published online: 19 December 2025
Original article
J of IMAB. 2025 Oct-Dec;31(4):6680-6686
THE RADICAL-SCAVENGING POTENTIAL AND OXIDATIVE STRESS MODULATION OF ROSA ALBA L. ABSOLUTE AGAINST GENTAMICIN-INDUCED RENAL TOXICITY
Silviya Zlateva1,2
, Kamelia Petkova-Parlapanska1, Anna Dobreva3, Zvezdelina Yaneva2, Тsvetelin Georgiev4, Petya Hadzhibozheva4, Ekaterina Georgieva1, Galina Nikolova1, Yanka Karamalakova1
,
1) Department of Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, Bulgaria.
2) Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.
3) Institute for Roses and Aromatic Plants, Agricultural Academy, Kazanlak, Bulgaria.
4) Department of Physiology, Pathophysiology and Pharmacology, Medical Faculty, Trakia University, Stara Zagora, Bulgaria.
ABSTRACT:
Purpose: This study aimed to evaluate the radical scavenging capacity and potential modulation of oxidative stress exerted by Rosa alba L. absolute in a model of gentamicin-induced nephrotoxicity.
Material/Methods: The antioxidant activity of R. alba L. absolute was examined using electron paramagnetic resonance (EPR) spectroscopy. In vivo EPR measurements were performed to assess scavenging activity against hydroxyl (•OH) and superoxide (•O2-) radicals, as well as the DPPH radical scavenging capacity (DPPH•). Tissue homogenates were prepared from renal samples and serum obtained from Balb/c mice. Animals were randomly allocated into four experimental groups (n = 6): (a) the control group, receiving an intraperitoneal injection (i.p.) of 1 mL isotonic NaCl solution (0.9%) daily along with a standard diet; (b) the R. alba group, administered R. alba absolute orally at 80 mg·kg-¹ body weight·day-¹; (c) the gentamicin (GM) group, injected i.p. with GM at 200 mg·kg-¹ body weight·day-¹ to induce acute nephrotoxicity; and (d) the combination group, receiving GM (200 mg·kg-¹) followed 2 hours later by R. alba absolute (80 mg·kg-¹·day-¹) administered orally. EPR spectroscopy was further employed to analyze oxidative alterations and conformational modifications in renal proteins, with a particular focus on sulfhydryl (-SH) group integrity in albumin. Protein carbonylation (PC) levels were quantified using an enzyme-linked immunosorbent assay (ELISA).
Results: The findings demonstrated that R. alba L. absolute exhibited pronounced antioxidant activity, effectively scavenging hydroxyl and superoxide radicals and displaying notable DPPH radical neutralization capacity.
Conclusions: Moreover, oral administration of R. alba significantly attenuated protein oxidation and carbonylation, thereby mitigating oxidative damage to renal proteins (p < 0.05).
Keywords: Rosa alba L., radical-scavenging potential, oxidative stress, gentamicin nephrotoxicity,
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Please cite this article as: Zlateva S, Petkova-Parlapanska K, Dobreva A, Yaneva Z, Georgiev T, Hadzhibozheva P, Georgieva E, Nikolova G, Karamalakov Y. The radical-scavenging potential and oxidative stress modulation of Rosa Alba L. absolute against gentamicin-induced renal toxicity. J of IMAB. 2025 Oct-Dec;31(4):6680-6686. [Crossref - 10.5272/jimab.2025314.6680]
Correspondence to: Prof. Yanka Karamalakova, Chemistry and Biochemistry Department, Medical Faculty, Trakia University; 11, Armeiska Str., 6000 Stara Zagora, Bulgaria; E-mail: yanka.karamalakova@trakia-uni.bg
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Received: 28 October 2025
Published online: 19 December 2025