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Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2007, vol. 13, book 1
Subject Collection: Medicine
Page: 24-26
DOI: 10.5272/jimab.2007131.24
Online date: September 10, 2007
J of IMAB 2007; 13(1):24-26
IMMUNOHISTOCHEMICAL INVESTIGATIONS OF P16 INK 4A EXPRESSION IN CARCINOMAS AND HIGH GRADE CERVICAL LESIONS
Milena Karcheva1, Savelina Popovska2, Rosen Nachev2
1) Department of Epidemiology of Infectious Diseases, Faculty of Public Health, Medical University - Pleven, Bulgaria
2) Department of General and Clinical Pathology, University Hospital-Pleven, Bulgaria
ABSTRACT:
During the years the role of viruses as a cause of oncological illness was known. One of them is cervical carcinomas, related to Human Papilloma Viruses (HPV's). There are about 450000 new cases of cervical cancer worldwide each year, and 250000 deaths of this diagnosis. By the diagnosis cervical cancer gets there with two procedures: after mass screening or clinical suspicious for ill. By the availability apparent tumor diagnosis is put with biopsy. By the discreet lesions require utilization modern diagnostic methods. One of them is immunohistochemical method. In situ hybridization (ISH) assays for high-risk human papillomavirus (HR-HPV) and immunohistochemical (IHC) assays for surrogate markers such as p16 INK 4a can be useful in detecting HR-HPV in cervical dysplasia. The aim of the following study was to find difference the level for expression of the tumor specific gene p16 INK 4a in reactive, dysplastic and invasive changes in biopsy specimens from uterine cervix. In the lesions of the uterine cervix, over expression of p16 INK 4a is induced by HPV and is associated with the process of cancerogenesis in the epithelium. Almost 100% of the high grade lesions and the invasive carcinomas demonstrated high level of expression of p16 INK 4a while non dysplastic epithelium is always negative.
Key words: cervical carcinomas, human papilloma viruses, p16 INK4a expression,
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Please cite this article as:
Karcheva M, Popovska S, Nachev R. Immunohistochemical investigations of P16 INK 4a expression in carcinomas and high grade cervical lesions. J of IMAB. 2007; 13(1):24-26. doi: 10.5272/jimab.2007131.24
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