 |
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2006, vol. 12, issue 1
Subject Collection: Medicine
Page: 3-6
DOI: 10.5272/jimab.2006121.3
Online date: November 4, 2006
J of IMAB 2006; 12(1):3-6
THE EFFECT OF ERBISOL ON PRODUCTION OF IL-1β AND NITRIC OXIDE (NO) BY CELLS OF IMMUNE SYSTEM IN PATIENTS WITH CHRONIC UROGENITAL CHLAMYDIOSIS IN VITRO.
Valentyna I. Fesenkova, Georgiy N. Drannik, V. E. Driyanska, V. V. Kologrimova
Ukrainian Research Center of Immunology Institute of Urology,
National Academy of Medical Science of Ukraine, Kyiv, Ukraine
ABSTRACT:
Infection caused by Chlamydia trachomatis is on the special place among sexually transmitted diseases, due to high incidence of chronic urogenital chlamydiosis (CUGC), social importance of the problem and difficulties in treatment. In spite of an active use of new medicaments, the number of CUGC patients continues to grow therefore the search for new measures of immunocorrection is also in progress.
Aim: to determine in vitro the functional activity of cells of monocyte-and-macrophage series in CUGC patients by the production of IL-1β and nitric oxide under the action of immunocorrecting medicament Erbisol.
Materials and methods: the cytokine production was determined through studying the spontaneous and induced synthesis of IL-1β by cells of CUGC patients in vitro by means of immunoenzymatic method employing the test system DIACLONE (France) and immunoenzymatic analyzer STAT-Fax Plus-303 (USA). The content of stable metabolite NO-ionic nitrite-NO2- in supernatants of cells of monocyte and macrophage series was determined by means of spectrophotometry method using the standart Greiss-reagent.
Results: the IL-1β production by cells of healthy donors was 44.9±1.29 pg/ml, while the meanindex of spontaneous IL-1β production in CUGC patients was statistically higher than in healthies – 156.5±6.2 pg/ml. The activation of immunocompetent cells by mitogen in patients led to statistical reducing of monokin production and averaged 119.0±5.0 pg/ml (p<0.05). But the addition of Erbisol to the patient’s immunocompetent cells stipulated the increase in IL-1β production almost 1,5 times, averaged 211.7±7.2 pg/ml and did not differ from the inducted cytokine production in healthy donors (p> 0.05). The spontaneous NO secretion in patients exceeded 2.5 times the NO level in healthy donors, the induction by Erbisol led to higher elevation of nitric oxide level in patients – about 3 times as compared with spontaneous production in healthies and 2 times exceeded the NO production induced by mitogen.
Conclusion: the lack of an increase in IL-1β production during activation by mitogens in CUGC patients in vitro, in contrast to healthies, can be considered as decrease in the reserve potential of cells of monocyte-macrophage series against the background of their high activation. Erbisol demonstrates the potential increase in IL-1β and NO production by patient’s cells, that can be considered as the positive effect for the perspective empoying of the medicament in a situation when during a long time the immune system cannot ensure the effective resistance and destroy pathogen-Chlamydia trachomatis.
Key words: IL-1β, nitric oxide, chronic urogenital chlamydiosis.
- Download FULL TEXT (PDF - 576 KB)
Please cite this article as:
Fesenkova VI, Drannik GN, Driyanska VE, Kologrimova VV. THE EFFECT OF ERBISOL ON PRODUCTION OF IL-1β AND NITRIC OXIDE (NO) BY CELLS OF IMMUNE SYSTEM IN PATIENTS WITH CHRONIC UROGENITAL CHLAMYDIOSIS IN VITRO. J of IMAB. 2006; 12(1):3-6; DOI:10.5272/jimab.2006121.3
REFERENCES:
1. Николаенко А.Н. Международная заявка РСТ/UА93/0003 "Биологически активное средство, способ его получения, препарат, содержавший указанное средство и способ использования препарата". 1993. [in Russian]
2. Битти В., Моррисон Р., Персистенция хламидий: от клеточных культур до патогенеза хламидийной инфекции. Заболевания передающиеся половым путем. - 1995. No. 6. C.3-24. [in Russian]
3. Малышев И.Ю. Введение в биохимию оксида азота. Роль оксида азота в регуляции основных систем организма. Рос. журнал гастроэнтерологии, гепатологии, колопроктологии. -1997.-No.1. С. 49-55. [in Russian]
4. Janeway CA, et al. Immunobiology: The Immune System in Health and Disease. New York, NY: Current Biology Publications; London, England: Elsevier Science London/Garland Publishing, 1999.
5. Dinarello CA. Interleukin-1. Rev Infect Dis. 1984 Jan-Feb;6(1):51-95. [PubMed]
6. Blesson S, Thiery J, Gaudin C, Stancou R, Kolb JP, Moreau JL, et al. Analysis of the mechanisms of human cytotoxic T lymphocyte response inhibition by NO. Int Immunol. 2002 Oct;14(10):1169-78.Rev Infect Dis. 1984 Jan-Feb;6(1):51-95. [PubMed] [CrossRef]
7. Igietseme JU, Uriri IM, Chow M, Abe E, Rank RG. Inhibition of intracellular multiplication of human strains of Chlamydia trachomatis by nitric oxide. Biochem Biophys Res Commun. 1997 Mar 27;232(3):595-601. [PubMed] [CrossRef]